壳聚糖固定化气单胞菌处理餐厨高油脂废水

Aeromonas immobilized on chitosan for treating high-oil wastewater from kitchens

为有效解决餐厨废水中的高油脂对下游处理工艺的严重影响,本研究以筛选获得的一株高效的油脂降解菌株嗜糖气单胞菌Aeromonas allosaccarophila CY-01为研究对象,以壳聚糖为载体材料,对其进行固定化包埋制备壳聚糖-气单胞菌小球(CH-CY01);探究CH-CY01小球的油脂降解效率以及性能影响评估。研究结果表明,经壳聚糖固定后的菌株CY-01其细胞的生长活性几乎不受影响,对大豆油脂的最大降解率为89.7%;相比于未固定的细胞,CH-CY01在0.5%NaCl的盐度条件下对油脂的降解效率显著提高了20%;在浓度为1 mg/L的表面活性剂(十二烷基苯磺酸钠)存在条件下,CH-CY01对油脂的降解效率显著提高了40%。以餐饮高油脂污水为处理对象,结果显示经添加1%(V/V)的CH-CY01小球处理7 d后,超过80%以上的固态油脂被降解,明显高于未固定的CY-01。综上所述,经壳聚糖固定包埋的CH-CY01小球显著提高了菌株的生存能力和油脂降解效率,对于高油脂餐饮污水的高效处理具有较大的应用潜力。

To effectively solve the serious impact of high oil in the kitchen wastewater on the downstream treatment process,an excellent oil-degrading strain Aeromonas allosaccarophila CY-01 was immobilized to prepare Chitosan-Aeromonas pellets(CH-CY01)by using chitosan as a carrier.Oil degradation condition and efficiency of CH-CY01 pellets were assessed.The growth of immobilized CH-CY01 was almost unaffected,and the maximum degradation rate of soybean oil was 89.7%.Especially at 0.5%NaCl concentration,oil degradation efficiency of CH-CY01 was increased by 20%compared with free cells.In the presence of a surfactant(sodium dodecylbenzene sulfonate)at 1 mg/L,the degradation efficiency of oil by CH-CY01 was increased by 40%.Moreover,using the high-oil catering wastewater as the substrate,more than 80%of the solid oil was degraded with 1%(V/V)CH-CY01 pellets treatment for 7 days,significantly higher than that of free cells.In summary,immobilized CH-CY01 significantly improved the efficiency of oil degradation.