摘要
核酸检测因具有良好的灵敏度和特异性而被广泛应用于体外诊断、动植物商品检疫、法医鉴定等领域。然而操作过程中易受到核酸污染导致的假阳性结果,严重影响了检测准确性。因此寻找一种有效的防止和清除核酸污染的方案对于实验室正常运转及保障检测结果的可靠性具有重要意义。文中比较了几种不同清除核酸污染的方法,确认了84消毒液和PCRguard试剂可有效清除液体中及不同材质表面的核酸污染。另外,84消毒液和PCRguard试剂配合使用,可很好地解决核酸气溶胶污染。研究结果对于分子诊断实验室日常预防核酸污染及清除已发生的气溶胶污染提出了解决方案。
Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis,animal and plant commodity quarantine,forensic identification,and other fields.However,it is susceptible to carryover contamination during the operation and leads to false-positive results,which seriously affects the detection accuracy.Therefore,finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent.This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials.Besides,the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination.This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.
作者
李云龙
张健
魏艳秋
贾晓娟
李小燕
谭立明
刘文军
杨利敏
Yunlong Li;Jian Zhang;Yanqiu Wei;Xiaojuan Jia;Xiaoyan Li;Liming Tan;Wenjun Liu;Limin Yang(Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;The Biological Safety level-3(BSL-3)Laboratory of Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;The Third Hospital of Qinhuangdao,Qinhuangdao 066000,Hebei,China;The Fifth Affiliated Hospital,Southern Medical University,Guangzhou 510900,Guangdong,China;Jiangxi Province Key Laboratory of Laboratory Medicine,Department of Clinical Laboratory Science,The Second Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2021年第2期673-679,共7页
Chinese Journal of Biotechnology
基金
国家科技重大专项(Nos.2016ZX10004222-006,2018ZX10734-404)
国家重点研发计划(Nos.2018YFC1200602,2018YFC1200502)资助。
关键词
核酸检测
PCR
气溶胶
核酸污染
nucleic acid detection
PCR
aerosol
nucleic acid contamination