万寿菊-烟草轮作调理植烟土壤细菌群落结构的作用

Effects of Marigold-Tobacco Rotation on Bacterial Community Structure in Tobacco Rhizosphere Soil

基于大田试验和Illumina Hiseq扩增子测序技术,分析了万寿菊-烟草轮作对植烟土壤细菌群落多样性和结构的影响,旨在为缓解烟草连作障碍提供理论依据。结果表明,万寿菊-烟草轮作可提高烟株根际土壤细菌群落Sobs、Shannon和Chao 1等多样性和丰富度指数;提升土壤中酸杆菌门(Acidobacteria)和拟杆菌门(Bacteroidetes)的相对丰度;促使生防菌(Pseudomonas)、功能菌(Candidatus_Solibacter、Bryobacter和Bradyrhizobium)、降解菌(Massilia、Novosphingobium和Ramlibacter)和根际促生菌(Flavisolibacter)等菌属比例上升,有利于改善土壤微生态环境、缓解连作障碍。

The research aims to provide a theoretical basis for alleviating the obstacles of tobacco continuous cropping.Field experiments and amplicon sequencing based on Illumina Hiseq were conducted to explore the effects of marigold-tobacco rotation on the diversity and structure of bacterial communities in tobacco rhizosphere soil.The results showed that marigold-tobacco rotation could greatly improve the Sobs,Shannon,Chao 1 indexes of soil bacterial communities.And,marigold-tobacco rotation can also improve the relative abundance of Acidobacteria and Bacteroidetes,and increase the proportion of biocontrol bacterium genera(Pseudomonas),functional bacteria genera(Candidatus_Solibacter,Bryobacter and Bradyrhizobium),degrading bacteria genera(Massilia,Novosphingobium and Ramlibacter)and plant growth promoting rhizobacteria(Flavisolibacter).In conclusion,marigold-tobacco rotation is strongly conducive to the improvement of soil microecological environment and alleviation of the obstacles of tobacco continuous cropping.