析因设计动物实验筛选聚桂醇硬化治疗肝囊肿最佳方案

Selection of lauromacrogol sclerotherapy for hepatic cyst:A factorial design experimental study

目的探讨聚桂醇在体硬化治疗肝囊肿兔模型的最佳药物浓度与作用时间组合方案。方法采用两因素三水平析因设计方法,将54只兔结扎胆囊管建成肝囊肿模型,并随机分为9组,每组6只,分别给予0.25%、0.50%及1.00%聚桂醇作用5、10及20 min。测量硬化治疗前及治疗后1周胆囊最大横径,评估硬化治疗效果;采用HE染色观察各组胆囊组织病理学变化。结果硬化治疗后1周,各组胆囊体积均缩小,囊壁全层均有不同程度变性坏死。以各组治疗后胆囊最大横径作为评价指标进行析因设计,结果显示聚桂醇浓度与作用时间对治疗效果存在交互影响(P=0.019)。两因素析因设计组合中,0.50%聚桂醇浓度作用10 min时K值最大(6.19)。结论聚桂醇在体硬化治疗肝囊肿兔模型的最佳药物浓度与作用时间组合方案为0.50%、10 min。

Objective To explore the optimal combination of concentration and action time of lauromacrogol in sclerotherapy of hepatic cyst in vivo rabbit models.Methods Two-factor three-level factorial design method was adopted.Fifty-four rabbit models of hepatic cyst established through ligation of cystic duct were randomly divided into 9 groups(each n=6).The rabbits were treated with 0.25%,0.50%,1.00%lauromacrogol for 5,10,20 min,respectively.The maximum transverse diameter of gallbladder was measured before and 1 week after treatment to evaluate the effect of sclerotherapy.Pathological changes of gallbladder tissue were observed after HE staining.Results One week after sclerotherapy,the volume of gallbladder reduced in all groups,and the whole wall of gallbladder had varying degrees of degeneration and necrosis.The maximal transverse diameter of gallbladder after treatment was used as the evaluation index for factorial design,and the results showed that there was an interaction between lauromacrogol concentration and action time of sclerotherapy(P=0.019).K value of the two-factor factorial design combination was the highest with 0.50%lauromacrogol for 10 min(K=6.19).Conclusion The best combination of drug concentration and action time of lauromacrogol for in vivo sclerotherapy of hepatic cyst rabbit models was 0.50%and 10 min.