青钱柳叶UPLC-Q-TOF-MS成分分析及其对睾丸的修复作用

Analysis of Cyclocarya Paliurus Leaves by UPLC-Q-TOF-MS and Its Effect on Testicular Restoration

目的研究青钱柳叶水提物的主要成分及其对糖尿病小鼠睾丸组织功能的影响。方法基于高分辨分子量和质谱碎片信息,结合数据库匹配,通过超高效液相色谱-四极杆-飞行时间-质谱联用(ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS)分析青钱柳叶水提物的组成成分,PNPG法测定α-葡萄糖苷酶抑制活性,并检测青钱柳叶水提物高(2000 mg·kg^(-1)·d^(-1))、中(1000 mg·kg^(-1)·d^(-1))、低(500 mg·kg^(-1)·d^(-1))剂量对四氧嘧啶诱导的糖尿病小鼠的治疗作用。结果从青钱柳叶水提物中鉴定出20个化合物,发现了3个新的α-葡萄糖苷酶抑制剂:桑色素[IC50=(3.98±1.38)μg·m L^(-1)]、7-羟基香豆素[IC50=(33.48±2.54)μg·m L^(-1)]和甲基丁香酚[IC50=(34.63±4.66)μg·m L^(-1)]。青钱柳叶水提物高、中、低剂量组与模型组相比,体质量和血糖值均有显著性差异,且睾丸组织切片显示高剂量组比阳性组有更好的修复效果。结论青钱柳叶水提取物中、高剂量有显著的降糖作用,对睾丸组织有较好的修复功能。

OBJECTIVE To analyze the chemical composition of the water extract of Cyclocarya paliurus leaves and its influence on testicular tissue in diabetic mice.METHODS The water extract of Cyclocarya paliurus leaves was analyzed by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS),and the chemical identification was then carried out based on the high-resolution molecular weight and fragment ion,as well as mass spectral database searching,theα-glucosidase inhibitory activity of some compounds was determined by PNPG method.Therapeutic effect of the Cyclocarya paliurus leaves on alloxan-induced diabetic mice was evaluated at doses of high(2000 mg·kg^(-1)·d^(-1)),medium(1000 mg·kg^(-1)·d^(-1))and low(500 mg·kg^(-1)·d^(-1)).RESULTS Twenty compounds were identified,and out of them,morin[IC50=(3.98±1.38)μg·m L^(-1)],umbelliferone[IC50=(33.48±2.54)μg·m L^(-1)],and methyleugenol[IC50=(34.63±4.66)μg·m L^(-1)]were first identified asα-glucosidase inhibitors from Cyclocarya paliurus leaves.There were significant different in body weight and blood glucose between the high,medium and low dose groups compared with the model group,and section diagram of testicular tissue showed that the high dose group has a better repair effect than the positive group.CONCLUSION The water extract of Cyclocarya paliurus leaves has significant hypoglycemic effect and good repair function to testicular tissue.