美廉净凝胶的抗炎、镇痛及抗瘙痒作用研究

Study on Anti-inflammatory,Analgesic and Anti-pruritus Effects of Meilianjing Gel

目的研究美廉净凝胶抗炎、镇痛及抗瘙痒的作用。方法通过蛋清液致小鼠足肿胀试验,羧甲基纤维素钠(carboxymethylcellulose sodium,CMC-Na)致小鼠皮下气囊白细胞游走试验,棉球致小鼠肉芽肿试验考察美廉净凝胶的抗炎作用;通过醋酸致小鼠扭体反应试验考察美廉净凝胶的镇痛作用;通过右旋糖酐-40致小鼠瘙痒试验考察美廉净凝胶抑制瘙痒的作用。结果美廉净凝胶各剂量组均可抑制蛋清液所致小鼠足趾肿胀;各剂量组均可降低小鼠致痒后30 min内的挠抓次数,但无统计学差异;高剂量组能显著抑制CMC-Na致炎后3 h的小鼠皮下气囊白细胞游走(P<0.05),高、中剂量组均能显著抑制CMC-Na致炎后6 h的小鼠皮下气囊白细胞游走(P<0.05);高剂量组可明显促进棉球诱导小鼠肉芽组织增生(P<0.01),且能降低醋酸致小鼠扭体的次数(P<0.05)。结论美廉净凝胶具有明显的抗炎、镇痛作用,且有一定的抗瘙痒效果。

OBJECTIVE To study anti-inflammatory,analgesic and anti-pruritus effect of Meilianjing gel.METHODS The anti-inflammatory effects of Meilianjing gel were investigated by the tests of using egg white solution to induce foot swelling in mice,carboxymethylcellulose sodium(CMC-Na)to induce leukocyte migration in subcutaneous balloon in mice,and cotton ball to induce granuloma in mice.The analgesic effect of Meilianjing gel was investigated by the test of acetic acid-induced writhing reaction in mice.The inhibition effect of Meilianjing gel on pruritus in mice induced by dextran-40 was investigated.RESULTS Each dose group of Meilianjing gel could inhibit the toe swelling of mice induced by egg white liquid.Each dose group could reduce the scratching frequency of mice within 30 min after itchiness,but there was no statistical difference.The high-dose group could significantly inhibit the leukocyte migration in the subcutaneous air sac of mice 3 h after the inflammation induced by CMC-Na(P<0.05),while the high-dose and medium-dose groups could significantly inhibit the leukocyte migration in the subcutaneous air sac of mice 6 h after the inflammation induced by CMC-Na(P<0.05).The high-dose group could significantly promote granulation tissue hyperplasia induced by cotton balls in mice(P<0.01),and the high-dose group could reduce the number of writhing induced by acetic acid in mice(P<0.05).CONCLUSION Meilianjing gel has obvious anti-inflammatory and analgesic effect,and has anti-pruritus effect.