从能力验证样品来探讨沙门氏菌的各种检测方法

Discussion on Various Detection Methods of Salmonella from Proficiency Testing Samples

目的通过对能力验证样品沙门氏菌的检验,探讨各种检测方法的特性,为食品、药品中沙门氏菌快速检测及鉴定提供参考。方法按照中国食品药品检定研究院下发的《能力验证作业指导书》,结合食品安全国家标准GB4789.4-2016和本实验室沙门氏菌常用检测方法进行平行检测,对样品进行分离、鉴定。结果 2份样品分别鉴定为鼠伤寒沙门氏菌和沙门氏菌Ⅲb(肠沙门氏菌双相亚利桑那亚种)。结论以国标、中国药典收载的传统方法为基础检测方法,加入简便快捷的其他检测方法作为辅助,可以确保结果的快速获得和准确性。

OBJECTIVE To explore the characteristics of various detection methods through the test of Salmonella in proficiency testing samples, so as to provide reference for the rapid detection and identification of Salmonella in food and medicine. METHODS According to the operation instruction for proficiency testing issued by National Institutes for Food and Drug Control, combined with the national food safety standard GB4789.4-2016 and the common detection methods of Salmonella in authors’ laboratory, the samples were isolated and identified. RESULTS The two samples were identified as Salmonella typhimurium and Salmonella Ⅲb(Salmonella enterica ssp. diarizonae). CONCLUSION Based on the traditional methods of GB and ChP, other simple and rapid detection methods are added to ensure the rapid acquisition and accuracy of the results.