暴马丁香成熟胚愈伤组织和体胚诱导及其生理状态解析

Callus Induction and Somatic Embryogenesis and Physiological State Analysis from Mature Zygotic Embryo Explant of Syringa reticulata var.mandshurica

以暴马丁香成熟胚为外植体进行愈伤组织和体胚发生诱导,通过调节诱导培养基中植物生长调节剂的种类和浓度,分析其对愈伤组织诱导和体胚诱导的影响,同时对培养过程中的外植体进行形态发生观察和生理状态分析。结果表明:①暴马丁香成熟胚外植体培养30 d可见直接体胚发生、60 d可见子叶型体胚;②BA在愈伤组织诱导过程中起到了主导作用,在0.5 mg·mL^(-1)BA和5~6 mg·mL^(-1)NAA组合下,愈伤组织诱导率可达100%;在0.5 mg·mL^(-1)BA和5 mg·mL^(-1)NAA组合体胚诱导率可达8%;③多酚含量在愈伤组织形成初期急剧上升且在培养过程中保持较高水平,子叶型胚期PAL和POD活性升高、MDA和SOD活性略下降。

In order to optimize somatic embryo induction system of Syringa reticulata var.mandshurica,callus induction and somatic embryogenesis from mature zygotic embryo were conducted by adjusting the type and concentration of plant growth regulators.The morphological observation and physiological analysis of the explants in the culture process were carried out.The results showed that:①Somatic embryo regenerate from mature zygotic embryo explant 30 d cultured later and cotyledonous somatic embryo can be obtained cultured 60 d later;②BA played a leading role in callus induction,the callus induction rate was reached 100%in the combinations of 0.5 mg·mL^(-1)BA with 5-6 mg·mL^(-1)NAA,and the somatic embryo induction rate reached 8%in the combination of 0.5 mg·mL^(-1)BA and 5 mg·mL^(-1)NAA;③Polyphenol content increased sharply at the primary stage of callus formation and kept a relatively high level in the process of culture.The PAL and POD activity increased and the MDA and SOD activity decreased in cotyledonous stage of somatic embryo.