胞外ATP对壳聚糖诱导的ROS和PAL活性变化的影响

Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity

以烟草BY-2悬浮细胞为材料,探讨了胞外ATP对壳聚糖引起的活性氧(reactive oxygen species,ROS)水平和苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)活性变化的影响。结果表明,5~20μg·mL^(-1)壳聚糖处理导致了烟草悬浮细胞细胞内ROS水平逐渐增加;壳聚糖也导致了PAL活性的增加,其活性在15μg·mL^(-1)壳聚糖处理下达到峰值,此后有所降低。10~40μmol·L^(-1)外源ATP处理未引起烟草悬浮细胞内ROS水平和PAL活性的显著变化。细胞外ATP水平则随壳聚糖浓度的增加而逐渐下降。本文进一步分析了细胞外ATP对壳聚糖引起的ROS水平和PAL活性变化的影响。结果显示,外源施加20μmol·L^(-1)ATP可以有效降低壳聚糖诱导的烟草悬浮细胞ROS水平上升,同时外源ATP也明显减缓了壳聚糖所诱导的PAL活性的上升。上述结果表明,细胞外ATP水平能够影响壳聚糖引起的ROS水平和PAL活性的变化。

In this experiment,tobacco BY-2 suspension cells were used as materials to investigate the effects of extracellular ATP on the chitosan-induced changes of ROS(reactive oxygen species)level and PAL(phenylalanine ammonia-lyase)activity.The results showed that the tobacco suspension cells mixed with chitosan 5-20μg·mL^(-1)increased the intracellular ROS level at adose-dependent manner,and the PAL activity also increased and reached to the maximum at 15μg·mL^(-1)chitosan,and then decreased slightly.There was not significant difference in ROS levels and PAL activity in tobacco suspension cells by adding 10-40μmol·L^(-1)exogenous ATP.The extracellular ATP(eATP)level gradually decreased with the concentration of chitosan increased.The effects of eATP on the chitosan-induced change of ROS level and PAL activity were further analyzed.The results showed that the exogenous ATP 20μmol·L^(-1)added to chitosan-induced tobacco suspension cells effectively alleviated the increases of ROS level and PAL activity in cells.These results showed that effects of extracellular ATP can affect the chitosan-induced changes in ROS level and PAL activity in cells.