基于调节外泌体释放电针对大鼠坐骨神经损伤后功能恢复的影响

Effect of Electroacupuncture on Functional Recovery of Rats after Sciatic Nerve Injury Based on Regulating the Release of Exosomes

目的观察抑制血清外泌体水平对电针治疗大鼠坐骨神经损伤的影响,探讨电针是否通过调节外泌体释放促进大鼠坐骨神经损伤后功能恢复。方法构建大鼠坐骨神经损伤模型,分组进行电针治疗和药物干预,采用GW4869腹腔注射抑制外泌体释放。观察大鼠患侧足一般情况,采用足迹分析评价坐骨神经功能指数,采用神经传导速度比和腓肠肌湿重比评价坐骨神经功能恢复情况,采用免疫荧光染色NF200、MBP分别标记轴突和髓鞘评价神经的再生修复情况。结果抑制血清外泌体水平后电针对大鼠坐骨神经损伤治疗效果受到影响。与模型组比较,电针组促进大鼠坐骨神经损伤后功能恢复效果显著;与电针组比较,电针+GW4869组大鼠足底溃疡严重,坐骨神经功能指数、坐骨神经功能恢复情况、神经再生修复情况均较差。结论电针通过调节外泌体释放可促进大鼠坐骨神经损伤后功能恢复。

Objective To observe the effects of inhibiting the level of serum exosomes on the treatment of rat sciatic nerve injury by electroacupuncture;To explore whether electroacupuncture can promote the functional recovery of rats after sciatic nerve injury by regulating the release of exosomes. Methods The sciatic nerve injury model rats were constructed, grouped for electroacupuncture treatment and drug intervention. Intraperitoneal injection of GW4869 was used to inhibit the release of exosomes. The general condition of the affected foot of the rats was observed;the footprint analysis was used to evaluate the sciatic nerve function index, the nerve conduction velocity ratio and the gastrocnemius wet weight ratio were used to evaluate the sciatic nerve function recovery;immunofluorescence staining of NF200 and MBP were used to label axons and myelin sheaths to evaluate nerve regeneration and repair. Results After inhibiting the level of serum exosomes, the efficacy of electroacupuncture on sciatic nerve injury in rats was affected. Compared with the model group, the electroacupuncture group had a significant effect on promoting functional recovery of rats after sciatic nerve injury. Compared with the electroacupuncture group, the electroacupuncture+ GW4869 group had serious plantar ulcers, and the sciatic nerve function index, sciatic nerve function recovery, and nerve regeneration and repair were poor. Conclusion Electroacupuncture can promote the functional recovery of rats after sciatic nerve injury by regulating the release of exosomes.