艾叶乙醇提取物对糖尿病小鼠血糖和血脂的影响

Effect of Artemisia Argyi Folium ethanolic extract on blood glucose and blood lipids in diabetic mice

为评价艾叶乙醇提取物对高脂饮食联合链脲佐菌素(STZ)诱导的糖尿病模型小鼠血糖血脂的影响,采用腹腔注射STZ(35 mg/kg)加高糖高脂饲料喂养ICR小鼠,建立糖尿病小鼠模型。糖尿病小鼠随机分成3组,模型组(5 mL/kg 0.5%CMC-Na)、艾叶乙醇提取物低剂量组(100 mg/kg)和高剂量组(400 mg/kg)。连续给药6周,每天记录小鼠的饮水量及摄食量,每周记录并比较小鼠的血糖指标。测定给药6周后小鼠血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密脂蛋白胆固醇(LDL-C)和糖耐量(OGTT)水平。结果显示,艾叶乙醇提取物高剂量组小鼠的饮水量、摄食量、体重及空腹血糖和血糖浓度-时间曲线下面积(AUC)明显降低(P<0.01),口服葡萄糖耐量也显著改善(P<0.01),艾叶乙醇提取物低剂量组小鼠的TG、TC和LDL-C显著降低(P<0.01)。实验结果提示艾叶乙醇提取物对糖尿病小鼠糖脂代谢具有显著调节、改善作用,且呈现一定的剂量依赖性。

To investigate the effect of ethanolic extract from Artemisia Argyi Folium on blood glucose and blood lipids in diabetic mice,ICR mice were induced by intraperitoneal injection of 35 mg/kg streptozotocin(STZ)and a high-carbohydrate/high-fat diet to construct type 2 diabetes mellitus model.Diabetic mice were randomly divided into three groups:the model group(5 mL/kg 0.5%CMC-Na),the Artemisia Argyi Folium ethanolic extract low-dose group(100 mg/kg)and high-dose group(400 mg/kg).During the treatment for 6 weeks,the amount of drinking water and food intake of mice were recorded every day.Blood glucose and body weight were recorded every week.After treatment for 6 weeks,serum total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),and oral glucose tolerance(OGTT)were measured.The results showed that the amount of drinking water and food intake of mice significantly decreased(P<0.01)in the Artemisia Argyi Folium ethanolic extract high-dose group;oral glucose tolerance was significantly improved(P<0.01)and the contents of TC,TG and LDL-C were significantly decreased in the Artemisia Argyi Folium ethanolic extract low-dose group(P<0.01).The ethanolic extract from Artemisia Argyi Folium could significantly improve the glucose and lipid metabolic disorder in T2DM mice in a dose-dependent manner.