玫瑰香味玫红百合和橙香味紫红花滇百合的花香成分研究

Study on the floral scent components of Lilium amoenum with rose fragrance and Lilium bakerianum var.rubrum with orange fragrance

采用顶空固相微萃取和气相色谱-质谱联用技术对5种不同香型百合花的挥发性物质进行定性和定量分析。结果表明:从具有玫瑰香味的玫红百合(Lilium amoenum)和橙香味的紫红花滇百合(L.bakerianum var.rubrum)这2种清香型野生百合中分别鉴定出33种和26种花香物质,从浓香型野生百合紫花百合(L.souliei)中鉴定出30种花香物质,从浓香型栽培百合‘西伯利亚’(‘Siberia’,O)和‘罗宾娜’(‘Robina’,OT)中分别鉴定出27种和34种花香物质。在清香型百合中,玫红百合的主要花香成分为芳樟醇(15.42%)、乙酸叶醇酯(9.93%)、桉油精(7.54%)、α-金合欢烯(α-法呢烯)(7.05%)等;紫红花滇百合的主要花香成分为芳樟醇(46.38%)、顺式-β-罗勒烯(33.08%)、β-月桂烯(4.75%)等。在浓香型百合中,紫花百合的主要花香成分为dl-柠檬烯(45.31%)、芳樟醇(13.57%)、苯乙醛(4.01%)等;‘Siberia’的主要花香成分为芳樟醇(48.53%)、苯甲酸甲酯(23.24%)、顺式-β-罗勒烯(13.99%)、异丁香酚(6.12%)等;‘Robina’的主要花香成分为顺式-β-罗勒烯(33.43%)、桉油精(11.93%)、苯甲酸甲酯(10.27%)、水杨酸甲酯(8.21%)、异丁香酚(7.67%)、芳樟醇(6.92%)等。5种百合的花香物质均以萜烯类为主,其中芳樟醇是5种百合共有的主要花香成分,苯甲酸甲酯、水杨酸甲酯等酯类在‘Siberia’和‘Robina’香气成分中相对含量较高。综上所述,玫红百合的玫瑰花香味可能与其香气成分中的α-金合欢烯有关,紫红花滇百合的橙香味可能与其香气成分中的β-月桂烯有关。

Headspace-solid-phase microextraction(HS-SPME)and gas chromatography-mass spectrometry(GC-MS)combined techniques were used to analyse qualitatively and quantitatively the volatile substances of five different types of lilies.The results showed as follows:33 and 26 kinds of floral scent components were identified from two light sweet fragrance wild lilies of Lilium amoenum with rose fragrance and L.bakerianum var.rubrum with orange fragrance,respectively;30 kinds of floral scent components were identified from wild L.souliei with strong smell;27 and 34 kinds of floral scent components were identified from the cultivars of‘Siberia’(O)and‘Robina’(OT)with strong smell,respectively.Among the lilies with light sweet fragrance,the main floral components of L.amoenum were linalool(15.42%),(Z)-3-hexen-1-ol,acetate(9.93%),eucalyptol(7.54%),α-farnesene(7.05%),etc;the main floral scent components of L.bakerianum var.rubrum were linalool(46.38%),Cis-β-ocimene(33.08%),β-myrcene(4.75%),etc.Among the lilies with strong smell,the main floral scent components of L.souliei were dl-limonene(45.31%),linalool(13.57%),benzeneacetaldehyde(4.01%),etc;the main floral scent components of‘Siberia’were linalool(48.53%),methyl benzoate(23.24%),Cis-β-ocimene(13.99%),isoeugenol(6.12%),etc;the main floral scent components of‘Robina’were Cis-β-ocimene(33.43%),eucalyptol(11.93%),methyl benzoate(10.27%),methyl salicylate(8.21%),isoeugenol(7.67%),linalool(6.92%),etc.Terpenes were the main type of floral scent components in the five lilies,and linalool was the main floral scent component in the five lilies.Methyl benzoate,methyl salicylate and other esters had relatively high contents in the‘Siberia’and‘Robina’.In conclusion,rose fragrance in L.amoenum may come fromα-farnesene,and orange fragrance in L.bakerianum var.rubrum may come fromβ-myrcene.