精子DNA损伤对体外受精胚胎移植的影响

Sperm DNA damage effects on in vitro fertilization embryo transfer

目的探讨不育男性患者其精子DNA损伤对体外受精-胚胎移植(IVF-ET)的胚胎质量及临床结局的影响。方法回顾性分析了在乌鲁木齐市妇幼保健院生殖中心行常规体外受精(IVF)和卵胞浆内单精子显微注射(ICSI)治疗的不孕夫妇,共计125例。按照精子DNA碎片指数(DFI)<15%、15%~30%、>30%为正常组、低损伤组、高损伤组,分别比较行IVF-ET治疗时不同组间正常受精率、正常卵裂率、优质胚胎率、囊胚形成率和临床妊娠率的差异。结果在IVF或ICSI治疗周期中,DFI<30%精子DNA损伤对正常受精率、正常卵裂率、优质胚胎率、临床妊娠率影响均差异无统计学意义(P>0.05)。在DFI>15%情况下,囊胚形成率有显著降低,差异具有统计学意义(P<0.05)。结论新鲜胚胎移植时,精子DNA损伤对IVF-ET结局的影响差异无统计学意义,对于DFI>15%的患者囊胚形成率有影响。

Objective To investigate the effects of sperm DNA damage on embryo quality and clinic outcome of IVF-ET.Method The data of 125 infertile couples who were treated with conventional in vitro fertilization(IVF)and intracytoplasmic sperm microinjection(ICSI)in the reproductive center of our hospital were analyzed retrospectively.The participants were divided into normal group,low injury group,and high injury group according to sperm DNA fragment index(DFI)<15%,15%-30%,and>30%,respectively.The normal fertilization rates,normal cleavage rates,high-quality embryo rates,blastocyst formation rates,and clinical pregnancy rates were compared among different groups treated with IVF-ET.Result In the cycle of IVF or ICSI treatment,there were no significant differences in the effects of DNA damage on normal fertilization rate,normal cleavage rate,high-quality embryo rate,and clinical pregnancy rate in the DFI<30%groups(P>0.05).In the DFI>15%groups,the blastocyst formation rates decreased significantly(P<0.05).Conclusion During fresh embryo transfer,sperm DNA damage had no significant effects on IVF-ET outcome but affected blastocyst formation rate in the patients with DFI>15%.