女性激素及相关受体与盆腔器官脱垂的相关性分析

Analysis on the correlations between female hormones,receptors and pelvic organ prolapse

目的探讨女性激素及其相关受体与盆腔器官脱垂(POP)的相关性,为临床诊治提供参考依据。方法选择2015年12月-2018年12月义乌市妇幼保健院19例POP患者作为观察组,选择无POP患者43例作为对照组,62例患者在入院时应用化学发光免疫分析法检测血清雌二醇(雌激素)、睾酮(雄激素)水平,采用HE染色观察两组盆底组织的胶原纤维情况,采用免疫组织化学法染色测定盆底组织(阴道壁、圆韧带及主、骶韧带)中雌激素、雄激素受体的表达。结果观察组与对照组女性血清雌激素、雄激激素水平比较,差异均无统计学意义(均P>0.05);观察组盆底组织平滑肌细胞内的胶原纤维明显低于对照组,盆底组织雌激素受体、雄激素的阳性表达强度也明显低于对照组。结论女性激素对盆底支持组织的作用主要由其受体表达水平介导发挥;女性激素与女性POP的发生密切相关。

Objective To explore the correlations between female hormones,receptors and pelvic organ prolapse(POP),provide a reference basis for clinical diagnosis and treatment.Methods From December 2015 to December 2018,19 patients with POP were selected from Yiwu Municipal Maternal and Child Health Care Hospital as observation group,43 patients without POP were selected as control group.Chemiluminescence immunoassay was used to detect the levels of serum estradiol and testosterone in the two groups.HE staining method was used to observe collagen fibers of pelvic floor tissue in the two groups.Immunohistochemical method was used to detect the expressions of estrogen receptor and androgen receptor in pelvic floor tissue(vaginal wall,round ligament,cardinal ligament,and sacral ligament).Results There was no statistically significant difference in serum estrogen and androgen levels between observation group and control group(P>0.05).The level of collagen fiber in smooth muscle cell of pelvic floor tissue in observation group was significantly lower than that in control group,the positive expression intensities of estrogen and androgen in pelvic floor tissue in observation group were significantly lower than those in control group.Conclusion The effect of female hormones on pelvic floor supporting tissue is mainly mediated by receptor expression level.Female hormone receptor is closely related to the occurrence of female POP.