子宫内膜癌miR-129-2甲基化与微卫星不稳定性的关系研究

Study on the relationship between miR-129-2 methylation and microsatellite instability in endometrial cancer

目的探讨子宫内膜癌患者miR-129-2甲基化状态与微卫星不稳定性的关系及其与子宫内膜癌临床病理特征间的关系。方法选取2017年3月-2019年3月温州市中心医院接受治疗的子宫内膜癌患者51例作为子宫内膜癌组,另选取因绝经期阴道不规则出血和月经紊乱需行子宫内膜病理检查者59例作为对照组,检测两组子宫内膜癌组织中miR-129-2基因启动子甲基化状态与微卫星不稳定状态,检测两者与子宫内膜癌临床病理参数相关性,检测miR-129-2基因启动子甲基化状态与微卫星不稳定状态之间相关性,采用Logistic分析影响子宫内膜癌发生危险因素。结果与对照组相比,子宫内膜癌组miR-129-2基因Cp G岛发生甲基化率、微卫星不稳定阳性率显著升高,差异有统计学意义(P<0.05);miR-129-2甲基化状态、微卫星不稳定状态与子宫内膜癌分化程度、临床分期、远处转移密切相关,差异有统计学意义(P<0.05);根据微卫星不稳定状态,将子宫内膜癌患者分成微卫星不稳定阴性组36例,微卫星不稳定阳性组15例,两组miR-129-2甲基化比例差异无统计学意义(P>0.05);Logistic分析显示,miR-129-2甲基化、微卫星不稳定是子宫内膜癌发生危险因素(OR=2.726,1.898,P<0.05)。结论子宫内膜癌组织中miR-129-2基因甲基化、微卫星不稳定性可能参与子宫内膜癌进程,miR-129-2基因甲基化与微卫星不稳定性无明显相关性。

Objective To investigate the relationship between the methylation status of miR-129-2 and microsatellite instability in patients with endometrial cancer and their relationships with clinicopathological characteristics of endometrial cancer.Methods 51 cases of endometrial cancer treated in our hospital from March 2017 to March 2019 were selected as endometrial cancer group.59 cases of endometrial pathology due to irregular vaginal bleeding and menstrual disorders during menopause were selected as control group.The methylation status and microlevel of promoter of microRNA-129-2 gene in endometrial cancer tissues of the two groups were detected.The correlation between microsatellite instability and the methylation status of promoter of microRNA-129-2 gene was detected.The risk factors of endometrial cancer were analyzed by Logistic analysis.Results Compared with the control group,the methylation rate and microsatellite instability positive rate of microRNA-129-2 gene Cp G island in endometrial cancer group increased significantly(P<0.05);MicroRNA-129-2 methylation and microsatellite instability were closely related to differentiation,clinical stage and distant metastasis of endometrial cancer(P<0.05).According to the microsatellite instability,endometrial cancer patients were divided into 36 cases of microsatellite instability negative group and 15 cases of microsatellite instability positive group;there was no significant difference in the ratio of microRNA-129-2 methylation between the two groups(P>0.05).Logistic analysis showed that mir-129-2 methylation and microsatellite instability were risk factors for endometrial cancer(OR=2.726,1.898,P<0.05).Conclusions:The miR-129-2 gene methylation and microsatellite instability may be involved in the process of endometrial cancer.There is no correlation between the methylation of miR-129-2 gene and microsatellite instability.