儿童支气管哮喘患儿外周血DC细胞数量和功能的分析研究

Analysis of the number and function of peripheral blood DC cells in children with bronchial asthma

目的观察支气管哮喘患儿外周血树突状细胞(DC)数量和亚型细胞的变化,研究DC细胞在支气管哮喘发病中的作用及可能的发病机理。方法 38例支气管哮喘患儿作为哮喘组,将哮喘组患儿随机分为控制组(21例)及新诊断组(17例),选取17例同期本院保健科体检健康儿童作为对照组,利用流式细胞技术先分选DCs细胞,后分选出亚型细胞:类浆细胞DC(p DC)和传统的DC细胞(c DC)。分析比较三组外周血c DC数量、DC功能指标[白细胞介素(IL)-12、外周血嗜酸性粒细胞比值(EOS%)、免疫球蛋白E(IgE)]。结果新诊断组p DC数量(14.19±6.89)×10^(6)/L高于控制组的(9.70±4.90)×10^(6)/L和对照组的(8.52±4.18)×10^(6)/L,差异均具有统计学意义(P<0.05);控制组与对照组比较,差异无统计学意义(P>0.05)。新诊断组c DC数量(3.85±1.32)×10^(6)/L与控制组的(4.16±1.51)×10^(6)/L和对照组的(3.67±1.85)×10^(6)/L比较,差异无统计学意义(P>0.05)。新诊断组的IL-12(28.65±10.20)pg/ml低于控制组的(31.23±7.93)pg/ml和对照组的(41.68±11.53)pg/ml,EOS%(12.3±4.6)%、Ig E(863±56)IU/ml高于控制组的(8.5±3.2)%、(231±38)IU/ml和对照组的(2.3±1.2)%、(32±16)IU/ml,且控制组的IL-12水平低于对照租,EOS%、Ig E水平均高于对照组,差异均具有统计学意义(P<0.05)。结论 p DC/c DC亚型漂移引起Th1/Th2免疫功能失调,p DC特性和功能异常启动免疫炎症反应可能在支气管哮喘的发生发展中产生了重要作用。

Objective To observe the changes in the number and subtypes of peripheral blood dendritic cells(DC)in patients with bronchial asthma,and study the role of DC cells in the pathogenesis of bronchial asthma and the possible pathogenesis.Methods There were 38 children with bronchial asthma selected as the asthma group.The children in the asthma group were randomly divided into control group(21 cases)and newlydiagnosed group(17 cases).17 healthy children who had physical examinations in the health department of the hospital during the same period were selected as the reference group.DCs were sorted by flow cytometry,and then the subtypes of DCs were selected:plasma like DC(p DC)and conventional DC(c DC).The peripheral blood c DC number,DC function indicators[interleukin(IL)-12,eosinophil ratio(EOS%)and immunoglobulin E(IgE)]were analyzed and compared among the three groups.Results The number of p DC(14.19±6.89)×10^(6)/L of newly-diagnosed group was higher than(9.70±4.90)×10^(6)/L of the control group and(8.52±4.18)×10^(6)/L of the reference group,and the difference was statistically significant(P<0.05).There was no statistically significant difference between the control group and reference group(P>0.05).The number of c DC(3.85±1.32)×10^(6)/L of newly-diagnosed group had no statistically significant difference compared with(4.16±1.51)×10^(6)/L of control group and(3.67±1.85)×10^(6)/L of reference group(P>0.05).The IL-12(28.65±10.20)pg/ml of newly-diagnosed group was lower than(31.23±7.93)pg/ml of the control group and(41.68±11.53)pg/ml of reference group,and EOS%(12.3±4.6)%and Ig E(863±56)IU/ml were higher than the control group[(8.5±3.2)%and(231±38)IU/ml]and reference group[(2.3±1.2)%and(32±16)IU/ml].The IL-12 of the control group was lower than the reference group,and EOS%and Ig E were higher than the control group,and the difference was statistically significant(P<0.05).Conclusion p DC/c DC subtype drift causes Th1/Th2 immune dysfunction,and the activation of immune inflammatory response by p DC characteristics and dysfunction may play an important role in the occurrence and development of bronchial asthma.