摘要
为了探明抗寒剂KHYE-1的叶喷施用方法及其对水稻秧苗抗寒性的影响。以早稻品种‘中嘉早17’为材料,用抗寒剂叶喷水稻秧苗并进行低温胁迫处理,用化学法、生化法测得生化数据。结果表明KHYE-1的最佳叶喷施用方法为叶喷浓度0.5%、叶喷次数为1次/天,连续3天、叶喷用量5.0 g/盒;在低温胁迫下,KHYE-1叶喷处理使秧苗N、P、K含量及叶绿素SPAD值维持较高水平,可溶性糖和脯氨酸分别比CK提高了147.4%和72.0%,过氧化物酶(POD)、过氧化氢酶(CAT)活性分别比CK增加了80.2%、2.63%。其整体效果优于ABA。抗寒剂KHYE-1能有效增强秧苗抵抗低温胁迫的能力。
The purpose is to study the foliar spraying application method of cold-resistant agent KHYE-1 and its effect on cold resistance of rice seedlings.The early rice variety‘Zhongjiazao 17’was used as material, the seedlings were treated with cold-resistant agent and cold stress, and the biochemical indexes of seedlings were further detected with chemical and biochemical method.The results showed that the best application method of KHYE-1 was spray concentration of 0.5%, one time per day for 3 days and spray dosage of 5.0 g/box.Compared with CK, the content of N(Nitrogen), P(Phosphorus), K(Potassium) and the value of chlorophyll SPAD in rice seedlings treated with cold-resistant agent under cold stress maintained at a relatively high level,and the content of soluble sugar and proline increased by 147.4% and 72.0%, and the activities of peroxidase(POD) and catalase(CAT) were 80.2% and 2.63% higher, respectively.In conclusion, the overall effect of KHYE-1 is better than that of ABA, and it could improve the cold-resistance of rice seedlings.
作者
任勃
王翰盈
付婷钰
文祝友
熊海蓉
张先文
Ren Bo;Wang Hanying;Fu Tingyu;Wen Zhuyou;Xiong Hairong;Zhang Xianwen(College of Resources&Environment,Hunan Agricultural University,Changsha 410128;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128;Chemistry and Materials Science,Hunan Agricultural University,Changsha 410128;College of Animal and Technology,Hunan Agricultural University,Changsha 410128)
出处
《中国农学通报》
2020年第33期1-6,共6页
Chinese Agricultural Science Bulletin
基金
科技部国家重点研发计划项目“双季稻全程机械化丰产节本增效关键技术”(2017YFD0301503),“双季稻病虫草害绿色防控与避灾减损关键技术”(2017YFD0301505)
作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目“植物源型抗寒剂提高水稻耐冷性状的分子机理研究”(19KFXM07)
湖南农业大学校级大学生研究性学习和创新性实验计划项目“水稻TCP21基因在胁迫响应过程中的表达和功能鉴定”(xcx19116)。
关键词
水稻
抗寒剂
叶面喷施
抗寒性
低温胁迫
rice
cold-resistant agent
foliar spraying
cold resistance
cold stress
