固定液和苏木精对动物生殖器官组织染色效果的影响

Fixative Fluid and Hematoxylin: Effects on Staining Animal Reproductive Organs

为了给不同动物生殖器官的组织学染色提供参考,以屠宰后收集到的小尾寒羊、藏羊及牦牛体内采集卵巢、睾丸、子宫角、附睾体等器官为试材,用石蜡切片技术及苏木精-伊红(H-E)染色方法,观察并比较使用不同固定液及不同的苏木精染色时间下各生殖器官的染色效果。结果显示,使用4%多聚甲醛固定36 h与使用AAF固定48 h的固定效果类似,但使用AAF固定完毕后的染色效果具有明显优势,而使用4%多聚甲醛固定后的染色时间缩短1/2。研究发现,通过不同固定液对各生殖器进行固定及调整苏木精染色时间,4%多聚甲醛和AAF固定液固定效果各不相同,经AAF固定液固定的组织所需固定时间及苏木精染色时间均长于使用4%多聚甲醛固定时间,且经AAF固定液固定后其染色效果具有明显优势。

To provide a basis for histological staining of different animal reproductive organs, the organs including ovary, testicle, uterine horn and epididymis were collected from small tailed han sheep, Tibetan sheep and yak after slaughtering as test materials, and paraffin sections and hematoxylin-eosin(H-E) staining methods were adopted to observe and compare the dyeing effects of reproductive organs with different fixation solution and hematoxylin staining time.Results showed that the fixation effect with 4% paraformaldehyde for 36 h was similar to that with AAF for 48 h, but the dyeing effect after fixing with AAF showed a distinct advantage, meanwhile the dyeing time after fixing with 4% paraformaldehyde was shortened by 1/2.The study found that, by fixing reproductive organs with different fixed fluids and adjusting the dyeing time of hematoxylin, fixation effects with 4% paraformaldehyde and AAF fixed liquid were different respectively.The time of both fixing the tissues with AAF fixed liquid and dyeing on them with hematoxylin were longer than that of the fixing time on tissue with 4% paraformaldehyde, and the dyeing effect with AAF fixed liquid demonstrated a distinct advantage.