青稞可溶性膳食纤维与姜黄素复配后增溶及促吸收作用研究

Study on solubilization and absorption enhancement of mixed soluble dietary fiber from Hordeum vulgare var.nudum and curcumin

目的青稞Hordeum vulgare var.nudum可溶性膳食纤维(soluble dietary fiber of Hordeum vulgare var.nudum,HVSDF)对姜黄素的增溶与促吸收作用研究。方法利用紫外分光光度法(ultraviolet spectrophotometry,UV)比较HV-SDF与姜黄素复配前后姜黄素在体外的溶出速率;利用超高效液相色谱串联三重四级杆质谱仪(UPLC-ESI-MS/MS)测定HV-SDF与姜黄素复配前后大鼠血浆中姜黄素及其葡萄糖醛酸化代谢产物(curcumin glucuronide,Cur-O-glu)的含量。结果HV-SDF和姜黄素复配物(HV-SDF-Cur)组的姜黄素的体外溶出度达到62.96%,而姜黄素组的体外溶出度只有26.42%,结果表明,HV-SDF复配姜黄素后可使姜黄素的体外累积溶出率明显增高;测定HV-SDF-Cur组大鼠血浆中姜黄素及Cur-O-glu的含量发现,其结果明显高于姜黄素组。结论HV-SDF与姜黄素复配后可以增加姜黄素的水溶性和生物利用度;同时可为中药难溶活性成分的临床应用提供新思路。

Objective To study the solubilization and absorption-promoting effect of soluble dietary fiber of Hordeum vulgare var.nudum(HV-SDF)on curcumin.Methods The dissolution rate of curcumin and HV-SDF-Cur was compared by ultraviolet spectrophotometry(UV).The content of curcumin and curcumin glucuronide(Cur-O-glu)in rat plasma of curcumin group and HVSDF-Cur group was determined by ultra high performance liquid chromatography tandem triple quadrupole mass spectrometer(UPLCESI-MS/MS).Results Dissolution rate of curcumin in the HV-SDF-Cur group reached 62.96%,while the curcumin group was only 26.42%.The cumulative dissolution rate of curcumin was significantly increased in HV-SDF-Cur group.The content of curcumin and Cur-O-glu in the plasma in the HV-SDF-Cur group was determined to be significantly higher than that of the curcumin group.Conclusion The soluble dietary fiber from H.vulgare var.nudum(HV-SDF)can increase the solubility and bioavailability of curcumin.This study can also provide new ideas for the clinical application of insoluble active ingredients of traditional Chinese medicine.