摘要
目的青稞Hordeum vulgare var.nudum可溶性膳食纤维(soluble dietary fiber of Hordeum vulgare var.nudum,HVSDF)对姜黄素的增溶与促吸收作用研究。方法利用紫外分光光度法(ultraviolet spectrophotometry,UV)比较HV-SDF与姜黄素复配前后姜黄素在体外的溶出速率;利用超高效液相色谱串联三重四级杆质谱仪(UPLC-ESI-MS/MS)测定HV-SDF与姜黄素复配前后大鼠血浆中姜黄素及其葡萄糖醛酸化代谢产物(curcumin glucuronide,Cur-O-glu)的含量。结果HV-SDF和姜黄素复配物(HV-SDF-Cur)组的姜黄素的体外溶出度达到62.96%,而姜黄素组的体外溶出度只有26.42%,结果表明,HV-SDF复配姜黄素后可使姜黄素的体外累积溶出率明显增高;测定HV-SDF-Cur组大鼠血浆中姜黄素及Cur-O-glu的含量发现,其结果明显高于姜黄素组。结论HV-SDF与姜黄素复配后可以增加姜黄素的水溶性和生物利用度;同时可为中药难溶活性成分的临床应用提供新思路。
Objective To study the solubilization and absorption-promoting effect of soluble dietary fiber of Hordeum vulgare var.nudum(HV-SDF)on curcumin.Methods The dissolution rate of curcumin and HV-SDF-Cur was compared by ultraviolet spectrophotometry(UV).The content of curcumin and curcumin glucuronide(Cur-O-glu)in rat plasma of curcumin group and HVSDF-Cur group was determined by ultra high performance liquid chromatography tandem triple quadrupole mass spectrometer(UPLCESI-MS/MS).Results Dissolution rate of curcumin in the HV-SDF-Cur group reached 62.96%,while the curcumin group was only 26.42%.The cumulative dissolution rate of curcumin was significantly increased in HV-SDF-Cur group.The content of curcumin and Cur-O-glu in the plasma in the HV-SDF-Cur group was determined to be significantly higher than that of the curcumin group.Conclusion The soluble dietary fiber from H.vulgare var.nudum(HV-SDF)can increase the solubility and bioavailability of curcumin.This study can also provide new ideas for the clinical application of insoluble active ingredients of traditional Chinese medicine.
作者
李锐
张倩
孙良
李岭慧
张心洁
朱宗萍
廖洋样
侯曙光
周睿
廖婉
LI Rui;ZHANG Qian;SUN Liang-kun;LI Ling-hui;ZHANG Xin-jie;ZHU Zong-ping;LIAO Yangyang;HOU Shu-guang;ZHOU Rui;LIAO Wan(State Key Laboratory of Traditional Chinese Medicine Resources with Southwestern Characteristics,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Chengdu Zhiyu Biological Technology Co.,Ltd.,Chengdu 610041,China;Taiji Group Sichuan Tai Chi Pharmaceutical Co.,Ltd.,Chengdu 610225,China;Chengdu Dongtang Zhixing Information Technology Co.,Ltd.,Chengdu 610041,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第2期349-356,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金面上项目(82073994)
四川省科技厅科技项目(2020JDJQ0049)
四川省科技厅科技项目(2016KZ0037)
四川省科技厅科技项目(2017NFP0038)
农业部杂粮加工重点实验室开放课题(2019CC02)
成都中医药大学杏林学者学科人才科研提升计划(XSGG2019005)
成都中医药大学杏林学者学科人才科研提升计划(QNXZ2018026)
教育部春晖计划(20191083-127)
四川省中医药管理局中医药开发专项(2018KF001)
四川省科技厅国际合作项目(2018HH0122)
成都市科技局国际科技合作项目(2017-GH02-00054-HZ)。
关键词
姜黄素
青稞
可溶性膳食纤维
复配
增溶
促吸收作用
溶解度
生物利用度
UPLC-ESI-MS/MS
curcumin
Hordeum vulgare L.var.nudum Hook.f.
soluble dietary fiber
formulation
solubilization
absorptionpromoting effect
solubility
bioavailability
UPLC-ESI-MS/MS