肺炎支原体感染患者外周血T细胞亚群 血清免疫球蛋白与患者病情的相关性研究

Peripheral blood T lymphocyte subsets and serum immunoglobulin levels in patients with Mycoplasma pneumoniae infection and their relationship with the disease activity

目的探讨肺炎支原体感染患者外周血T淋巴细胞亚群、血清免疫球蛋白与患者病情的相关性。方法取2019年1月至12月因肺炎支原体感染来我院接受治疗的68例患儿为本次研究组,并选取同时期来我院体检的68名健康者为对照组。分别抽取2组人员空腹静脉血,经10 min离心操作后取其上清液,分别监测其实验室指标、外周血T细胞亚群与血清免疫球蛋白指标,并比较其检测结果。结果与对照组比较,研究组超敏C-反应蛋白(hs-CRP)及降钙素原(PCT)指标均较高(P<0.05),2组的白细胞计数、血小板计数(PLT)及红细胞沉降率(ESR)指标差异无统计学意义(P>0.05);与对照组比较,研究组CD8+水平较低,而CD4+/CD8+及总T细胞水平则较高(P<0.05),2组CD4+水平差异无统计学意义(P>0.05);与对照组比较,研究组IgG水平显著较高(P<0.05),而2组IgM及IgA指标则差异无统计学意义(P>0.05);与恢复期比较,急性期患儿的CD4+及IgA指标均较低,而IgG与IgM指标则较高(P<0.05)。结论肺炎支原体感染患者大多存在免疫功能减退与紊乱情况,外周血T细胞亚群与血清免疫球蛋白动态监测,能够对患者病情严重程度进行准确判定,具有较高的临床应用价值。

Objective To investigate how the peripheral blood T lymphocyte subsets and serum immunoglobulin levels in patients with Mycoplasma pneumoniae infection relates to disease activity.Methods A total of 68 children treated for Mycoplasma pneumoniae infection in our hospital between January 2019 and December 2019 were included as the study group.A contemporary cohort of 68 health check-up children were recruited as the control group.Fasting venous blood samples were collected from the two groups and subjected to 10-min centrifugation;thereby the supernatant was obtained and examined for various laboratory parameters,peripheral blood T-lymph subsets and serum immunoglobulin levels.The findings were compared between groups.Results Compared with the control group,the study group showed lower levels of hs-CRP and PCT(P<0.05),but there were no significant difference in WBC,PLT and ESR(P>0.05).Compared with the control group,the study group showed lower level of CD8+,higher CD4+/CD8+ratio and total T lymphocyte counts(P<0.05),but the two groups did not differ significantly in CD4+levels(P>0.05).Compared with the control group,the study group had significantly higher IgG level(P<0.05),but there were no significant differences in IgM and IgA between the two groups(P>0.05).Compared with convalescent subjects,children during acute stage of the disease had lower levels of CD4+and IgA,and higher levels of IgG and IgM(P<0.05).Conclusion Patients with Mycoplasma pneumoniae infection mostly have diminished or perturbed immunity.Dynamic monitoring of peripheral blood T-lymph subsets and serum immunoglobulins may help in accurate assessment of the disease activity and decision on proper,specific treatment strategies,and therefore is clinically useful.