小儿治哮灵片对幼年大鼠哮喘的干预作用

Effect of Xiaoer Zhixiaoling Tablets on asthmatic model of young rats

目的研究小儿治哮灵片对幼年大鼠哮喘模型的干预作用及机制。方法36只雄性Wistar幼年大鼠随机分为6组:对照组、模型组、醋酸地塞米松(阳性药,1 mg/kg)组和小儿治哮灵片低、中、高剂量(0.32、0.64、1.28 g/kg)组。除对照组外,其余各组以卵清蛋白(OVA)致敏、激发制备幼年大鼠哮喘模型。造模第8天开始ig给药,每天给药1次,给药至造模3周,对照组和模型组ig给予蒸馏水。记录各组大鼠引喘潜伏期并进行行为学评分;检测肺脏器指数;酶联免疫吸附法(ELISA)检测各组幼年大鼠血清中白细胞介素(IL)-4、IL-5、IL-6、免疫球蛋白E(IgE)、γ-干扰素(IFN-γ)、核转录因子(NF-κB)的水平以及肺泡灌洗液中IFN-γ水平。结果与模型组比较,小儿治哮灵片高、中、低剂量组引喘潜伏期及哮喘行为评分均显著下降(P<0.05、0.01);肺脏器指数显著下降(P<0.05);血清IL-4、IL-5、IL-6、IgE、NF-κB水平均显著下降(P<0.01);血清与肺泡灌洗液中IFN-γ水平显著升高(P<0.01)。结论小儿治哮灵片抗哮喘作用可能与降低炎性细胞因子分泌有关。

Objective To study the interventional effect and mechanism of Xiaoer Zhixiaoling Tablets on asthmatic model in juvenile rats.Methods Thirty six young male Wistar rats were randomly divided into six groups:control group,model group,dexamethasone acetate(positive drug,1 mg/kg)group and low,medium and high dose(0.32,0.64,1.28 g/kg)groups of Xiaoerzhixiaoling Tablets.Expect the blank control group,the other groups of young rats were sensitized by egg albumin(OVA)and stimulated to prepare the rat model of asthma in juvenile rats.On the 8 th day of modeling,drugs were ig given once a day until 3 weeks for modeling.The control group and model group were given distilled water.The incubation period of asthma was recorded and the behavior score was evaluated.The levels of IL-4、IL-5、IL-6、IgE、IFN-γ、NF-κB in serum of young rats and the level of IFN-γin alveolar lavage were detected by enzyme-linked immunosorbent method.Results Compared with the model group,the asthma latency and asthma behavior scores in the high,middle and low dose groups of Xiaoer Zhixiaoling Tablets were significantly decreased(P<0.05 and0.01),and the lung organ index was significantly decreased(P<0.05);levels of IL-4,IL-5,IL-6,IgE,NF-κB decreased in the highdose group,the medium-dose group and the low-dose group(P<0.01),and levels of IFN-γin serum and alveolar lavage fluid increased(P<0.01).Conclusion The anti-asthma effect of Xiaoer Zhixiaoling Tablets;may be related to the decreased secretion of downstream inflammatory cytokines.