HPLC法测定美洲大蠊药材中五种核苷类成分含量

Determination of Five Nucleosides in Periplaneta Americana L.Extract by HPLC

目的用HPLC法测定美洲大蠊药材中五种核苷类成分含量。方法用石油醚对美洲大蠊粉末进行脱脂,以65%的乙醇为溶剂进行超声提取,乙腈溶解配制成美洲大蠊供试品溶液,使用Agilent色谱柱(9.4×250 mm;5μm),以纯水∶乙腈=95∶5为流动相,流速为0.5 mL/min,检测波长为254 nm,进样量取10μL,对照混合标品和单一标品对美洲大蠊供试品溶液进行含量测定。结果用HPLC法测定美洲大蠊药材中五种核苷类成分含量,灵敏度高,专属性好,可用于本品的含量测定。5种核苷浓度在一定范围内与峰面积呈良好线性关系(R^(2)>0.9941),精密度高,重复性好,稳定性佳,加样回收率均在97.58%~103.26%之间。结论该法实验过程简便、结果可靠、稳定,可用来测定美洲大蠊中核苷类成分的含量。

Objective To determine the content of five nucleosides in Periplaneta Americana L.extract by HPLC.Methods We did degreasing the Periplaneta Americana L.powder with petroleum ether,ultrasonic extraction of 65%ethanol as solvent,acetonitrile dissolution formulated into the American dating test solution,The Agilent alkyl silica gel column(9.4 mm×250 mm,5μm)was applied in HPLC determination with water and acetonitrile mixture(95∶5)as the mobilephase at the flow rate of 0.5 mL•min^(−1).Detection wavelength was set at 254 nm,10μL sample was injected for analysis.Results The determination of five nucleosides in Periplaneta Americana L.extract by HPLC with high sensitivity,special properties,could be used for the content determination of this product.The concentration of 5 kinds of nucleoside in a certain range and peak area showed a good linear relationship(R^(2)>0.9941),high precision,good repeatability,good stability,plus sample recovery rate is between 97.58%to 103.26%.Conclusion The experimental process of this method is simple,the results are reliable and stable,and it can be used as a method for determining the content of nucleoside components in Periplaneta Americana L.