新型单细胞分析技术——质谱流式细胞术在白血病治疗中的应用

Application of New Single-cell Analysis Technology——Cytometry by Time-of-flight in Treatment of Leukemia

质谱流式细胞术(CyTOF)是结合质谱原理和流式技术的新型单细胞分析技术,近年来,CyTOF以其多参数、高通量、无需补偿计算及多元化数据分析等技术优势,在白血病治疗的研究中广泛应用,可精确测定细胞中元素含量,可在单细胞水平分析白血病细胞独特表型与复杂的信号通路的变化,用于研究抗白血病药物的作用机制、发现白血病潜在治疗靶点、评估治疗反应、深入了解白血病复发和耐药机制。本文就CyTOF原理、特点、优势及其在白血病治疗中应用的研究进展予以综述。

Cytometry by time-of-flight(CyTOF)is an innovative single-cell analysis technique combining with mass spectrometry principles and flow cytometry.In recent years,CyTOF has been widely used in the study of leukemia treatment for its advantages of multi-parameter,high-throughput,compensation without calculation and diversified data analysis.It can accurately determine intracellular element contents and analyze the changes of unique phenotype and complex signaling pathways of leukemia cells at the single-cell level,to study the mechanism of action of anti-leukemia drugs,discover the potential therapeutic targets of leukemia,assess the therapeutic response and deeply understand the mechanism of leukemia relapse and drug resistance.This article reviews the research progress of CyTOF principle,characteristics,advantages and its application in leukemia treatment.