食品中常见病原微生物的DNA条形码识别技术

Study on DNA barcoding identification technology of common pathogenic microorganisms in food

为探究DNA条形码技术在微生物识别方面的应用前景,对食品中常见的8种病原微生物的16S rRNA序列进行了比对,设计通用引物后,进行PCR扩增、测序及数据库检索,尝试建立一套基于DNA条形码的微生物识别方案。结果表明,扩增的V3~V6可变区域能准确区分变形杆菌纲(肠杆菌目、弧菌目、假单胞菌目)的致病菌和相关干扰菌,对芽孢杆菌纲(芽孢杆菌目)可鉴定到“属”水平。

In order to explore the application prospects of DNA barcode technology in microbial identification,the 16S rRNA sequences of 8 common pathogenic microorganisms in food were compared.The PCR amplification,sequencing and database search were conducted followed by universal primers designing,which were purposed to establish a microbial identification method based on DNA barcode.The results showed that the variable region sequence of V3~V6 amplified provided sufficient resolution for Gammaproteobacteria Enterobacteriales,including Vibrionales,and Pseudomonadales to accurately distinguish pathogenic bacteria and related interfering bacteria,corresponding to Bacillibacteria(Bacillales)could be identified to the“genus”level.