基于单链DNA模板合成铜纳米簇的四环素无标记检测

SsDNA-templated fluorescent copper nanoclusters for label free detection of tetracyclines

以聚T单链DNA为模板合成的铜纳米簇作为荧光探针,建立了一种快速、非标记型的荧光生物传感检测新方法,并用于实际样品中四环素的检测。结果表明,该方法用于检测四环素残留的检出限为0.01μmol/L,用于实际样品四环素残留的测定低限为48μg/kg,而且无需要任何荧光染料标记或复杂的DNA序列设计,所有过程均在室温下10 min内完成。将该方法应用于牛奶、鸡肉样品中四环素含量的测定,相对标准偏差≤4.01%,加标回收率为97.67%~110.23%。

In this paper,a novel and label-free fluorescence sensing strategy has been developed for tetracyclines(TCs)detection in food sample by using poly(thymine)(poly T)-templated copper nanoclusters(CuNCs)as fluorescent probes.Under optimized conditions,the label-free fluorescent sensor achieved highly sensitive and selective detection of TCs with a detection limit of 0.01μmol/L,and with a detection limit of 48μg/kg in real samples.Furthermore,the method was successfully applied to the detection of TCs in milk and chicken samples.The relative standard deviation was less than 4.01%,and the recovery rate was between 97.67%and 110.23%.The strategy was free of any fluorescence dye label or complex DNA sequence design,and all the processes were performed within 10 minutes at room temperature.Therefore,it could offer a simple,rapid,and label-free sensing platform for fluorescence detection of TCs in food.