摘要
本文通过多次催化酯交换方法,研究了最优催化条件下脂肪酶活性和结构的关系,以及低活性脂肪酶的活性再生技术。在催化合成1,3-二油酸-2-棕榈酸甘油三酯的研究中,利用荧光光谱和傅里叶变换红外光谱,分析最优条件下不同使用次数脂肪酶的三级与二级结构,研究脂肪酶活性变化与其二级、三级结构之间的关系。使用异丙醇和混合油脂处理脂肪酶,使低活性脂肪酶的活性得以恢复。研究结果表明:随着脂肪酶活性的降低,脂肪酶荧光强度增强,氨基酸暴露面积逐渐增大,在二级结构中,α-螺旋含量降低,β-折叠含量逐渐增加。通过异丙醇处理,脂肪酶活性恢复到原脂肪酶的75.00%;再经混合油脂处理,脂肪酶活性恢复到原脂肪酶的96.60%,实现了高效催化恢复活性的目的。
In this experiment,through multiple catalytic transesterification methods,the relationship between lipase activity and structure under optimal catalytic conditions and the activity regeneration technology of low-activity lipase are studied.Fluorescence spectroscopy and Fourier transform infrared spectroscopy were used to analyze the tertiary and secondary structures of lipases and the relationship between changes in lipase activity and its secondary tertiary structure.Low-activity lipases were restored by treating lipase with isopropanol and mixed fats.The study results showed that with the decrease of lipase activity,the fluorescence intensity of lipase increased gradually,and the amino acid leakage was more and more.In the secondary structure,theα-helix content decreased and theβ-sheet content increased gradually.Through isopropanol treatment,the lipase activity was restored to 75.00%of the original lipase;through the mixed oil treatment,the lipase activity was restored to 96.60%of the original lipase,realizing the purpose of high-efficiency catalytic restoration.
作者
王致禹
陈晓倩
孟庆凤
李春
刘丽波
曲国强
孙雪婷
Wang Zhiyu;Chen Xiaoqian;Meng Qingfeng;Li Chun;Liu Libo;Qu Guoqiang;Sun Xueting(College of Food Science and Technology,Northeast Agricultural University,The Key Laboratory of Dairy Science,Ministry of Education,Harbin 150030;Jiaotie Inpection and Certification Center(Chengdu)Co.,Ltd.,Chengdu 610000)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第2期55-62,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金面上项目(31671874)
国家重点研发计划项目(2018YFD0502404)
黑龙江省科学基金项目(C2018022)。
关键词
脂肪酶
二级结构
三级结构
活性再生
傅里叶红外光谱
lipase
secondary structure
tertiary structure
activity recovery
Fourier transform infrared spectroscopy(FTIR)