负载葡萄皮粉花色苷提取物的W/O/W型复乳制备及其体外消化评价

Formation and in Vitro Digestion of W/O/W Emulsions Loading Anthocyanin Extract from Grape Skin Powder

以葡萄皮花色苷(ACNs)提取物为内水相(W1),玉米油为油相,乳清蛋白(WPI)溶液为外水相(W2)制备水包油包水(W/O/W)型复乳。复乳的包埋率高达(93.19±2.78)%,平均粒径为(287.90±3.12)nm。经口腔消化后,乳液未发现有明显变化;模拟胃部消化后,乳液液滴相互融合形成具有双层结构的较大微粒,其平均粒径(d>600 nm)显著增加(P<0.05)。经模拟肠道消化后,微粒成为空油滴,其平均粒径为(387.53±15.96)nm,此时抗氧化活性最高。研究结果表明,W/O/W型复乳在口腔和胃部能有效保护ACNs,实现靶向性肠道输送。负载ACNs的复乳系统在食品工业中有很大的应用潜力。

In present study,a water-in-oil-in-water(W/O/W)double microemulsions were prepared with the water-extracted anthocyanins(ACNs)from grape skin as the inner water phase(W1),corn oil as the oil phase,and whey protein isolate(WPI)solution as the outer water phase(W2).The double emulsions showed high encapsulation efficiency of(93.19±2.78)%,and their droplet diameters averaged(287.90±3.12)nm.After mouth digestion,no obvious morphological change in the droplets was observed.Under the simulated gastric conditions,the droplets fused with each other to form larger droplets with double-layer structure,and the droplet sizes(d>600 nm)increased significantly(P<0.05).During simulated intestinal digestion,the double droplets became empty oil droplets with average droplet sizes of(387.53±15.96)nm,and the highest antioxidant activities were also identified in this stage.Our results indicated that W/O/W emulsions effectively protected ACNs against simulated mouth and gastric digestion,and targetedly delivered to the small intestine.Furthermore,the microcapsule system loaded with ACNs could have huge application potential in food industry.