摘要
组胺(HA)是影响人类健康和食品质量的重要生化物质。对其进行快速、准确检测和鉴定至关重要。本试验利用氧化石墨烯对单链寡核苷酸(ssDNA)的强吸附作用,分离与靶标结合和未结合的ssDNA,对组胺适配体进行11轮的体外指数富集筛选,最终得到15条高亲和力和特异性的适配体。通过后续的适配体二级结构、亲和性和特异性分析,HAA-4和HAA-7被确定为最佳候选适配体,可被作为将来关于组胺的新型检测方法构建的候选适配体。本文首次利用氧化石墨烯指数富集(GO-SELEX)技术筛选出对组胺具有较高亲和特异性的适配体,为后续利用适配体对食品中组胺快速检测奠定了基础。
Histamine(HA)is an important biochemical substance that affects human health and food quality.It is essential to develop a rapid and accurate system for HA detection and identification.In this study,the SELEX method was applied to select single-stranded(ss)DNA aptamers with high binding affinity and specificity to HA.Graphene Oxide(GO)was used to separate the unbound ssDNA,due to its strong adsorption ability to unbound ssDNA.After 11 rounds of selection and counter-selection,15 high-affinity and specific aptamers were obtained.Further analysis indicated that HAA-4 and HAA-7 were the best candidate aptamers by subsequent aptamer secondary structure,affinity and specificity analysis.The present study demonstrated the feasibility to screen HA aptamers by GO-SELEX,which would pave the way for its application in rapid detection of HA in food.
作者
朱勤超
冯俊丽
戴志远
汪艺
Zhu Qinchao;Feng Junli;Dai Zhiyuan;Wang Yi(Institute of Seafood,Zhejiang Gongshang University,Hangzhou 310012;Key Lab.of Aquatic Processing of Zhejiang Province,Hangzhou 310012)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第2期334-340,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省科技计划项目(2017C32072)。
