Capture-SELEX技术筛选β-苯乙胺核酸适配体

Selection of DNA Aptamers againstβ-phenethylamine Using Capture-SELEX

利用指数富集配体的系统进化技术(SELEX)中基于固定ssDNA文库的捕获SELEX(Capture-SELEX)技术,筛选能特异性识别β-苯乙胺的核酸适配体。通过在Capture-SELEX技术中结合熔解曲线监测ssDNA文库的富集,并经过氧化石墨烯(GO)荧光法验证候选适配体的亲和力和特异性,证实获得一条特异性识别β-苯乙胺的适配体(PHE-2),解离常数(Kd)为(71.64±11.47)nmol/L。该适配体为β-苯乙胺的分析检测提供了新的分子识别元件。

In this study,we described the selection of DNA aptamers forβ-phenethylamine using Capture-SELEX based on fixed ssDNA library,which is the technology of systematic evolution of ligands by exponential enrichment(SELEX).During the Capture-SELEX progress,melting curve analysis was developed to monitor the enrichment of the aptamer species.The affinity and specificity of the aptamer were verified by graphene oxide(GO)fluorescence method.We obtained an aptamer(PHE-2)ofβ-phenethylamine with a Kd value of(71.64±11.47)nmol/L.The aptamer provides a new molecular recognition element for the analytical detection ofβ-phenethylamine.