异黄芪甲苷体外对淋巴细胞和树突状细胞免疫功能的影响

Effect of iso-astragalosideⅥon immunologic function of lymphocytes in vitro and dendritic cells

目的研究异黄芪甲苷(6-O-β-D-葡萄糖基-环黄芪醇)对体外淋巴细胞和树突状细胞功能的影响。方法培养巨噬细胞,观察异黄芪甲苷对细胞吞噬中性红能力和白细胞介素-1(interleukin-1,IL-1)、IL-2、IL-6分泌功能的影响。MTT法检测异黄芪甲苷对原代培养脾淋巴细胞增殖的影响,并用流式细胞术通过检测CD3、CD19水平,明确异黄芪甲苷分别对T、B淋巴细胞增殖的影响。进一步观察异黄芪甲苷对树突细胞增殖和分泌IL-6、IL-12 p70能力的影响。结果异黄芪甲苷低、中、高3个剂量组可升高巨噬细胞的吞噬功能和分泌IL-6的能力,降低CD3^(+)的比例,升高CD19^(+)的比例,纠正T、B淋巴细胞的紊乱。中、高剂量组可显著升高IL-2水平,增加淋巴细胞的增殖,提高增殖指数(SI),促进树突状细胞(dendritic cells,DC)分泌IL-6和IL-12p70。高剂量组还可升高巨噬细胞分泌IL-1的能力。结论异黄芪甲苷的免疫促进作用机制与促进非特异性免疫吞噬,促进抗原递呈、淋巴细胞增殖和树突细胞分泌能力增强有关。

Objective To study the effect of iso-astragalosideⅥ(6-O-β-D-glucosyl-cycloastragenol)on the function of lymphocytes in vitro and dendritic cells.Methods The macrophages were cultured,and the effects of iso-astragaloside VI on helping to devour neutral red and secrete IL-1,IL-2 and IL-6 were also observed.Its effects on proliferating of primary cultured spleen lymphocytes were measured,along with the measurements of CD3 and CD19 by flow cytometry to determine its effects on T and B lymphocytes proliferations.In addition,its effects on promoting the proliferation and secretion of IL-6 and IL-12 p70 in dendritic cells(DC)were also observed.Results The three groups(low,medium and high doses)of iso-astragalosideⅥincreased the abilities of macrophages on phagocytosis and secretion of IL-6,improved T and B lymphocytes ratios by decreasing CD3^(+)and enhancing CD19^(+).Medium and high doses of iso-astragaloside VI elevated IL-2 level,lymphocyte proliferation and SI and promoted secretion of IL-6 and IL-12 p70 from DC.Additionally,the high dose group had the ability to increase the production of IL-1.Conclusion The potential mechanisms of iso-astragaloside VI on immunoenhancement can promote nonspecific immune phagocytosis,antigen presentation,lymphocyte proliferation and DC secretion.