基于UPLC指纹图谱结合化学计量学评价不同产地盾叶薯蓣药材质量

Quality evaluation of Dioscorea zingiberensis from different origins based on UPLC fingerprint and chemometrics

目的建立盾叶薯蓣Dioscorea zingiberensis的UPLC指纹图谱,分析不同产地盾叶薯蓣质量特征的共有性和差异性,为盾叶薯蓣药材的质量评价提供科学依据。方法采用色谱柱Infinity Lab Poroshell 120 EC-C18(150mm×2.1mm,2.7μm),以乙腈(B)-水(A)为流动相梯度洗脱,体积流量0.3mL/min,检测波长203nm,柱温30℃,建立65批不同产地盾叶薯蓣药材的UPLC指纹图谱;利用SPSS19.0和SIMCA14.1软件对不同产地的盾叶薯蓣药材进行质量评价及差异性分析。结果盾叶薯蓣药材UPLC指纹图谱共标定31个共有峰,指认出的5种皂苷类成分和其他未知成分均作为主要信息参与了盾叶薯蓣的质量表达,主成分载荷值的综合得分表明不同产地盾叶薯蓣药材的综合质量差异较大,主成分分析(PCA)结果显示不同产地盾叶薯蓣的化学质量特征存在差异,且各自聚为一类,其中湖北丹江口地区盾叶薯蓣与其他产地样品均存在较大差异;偏最小二乘法分析(PLS-DA)模型筛选出的5、28、11、12、29、18、16、31、13号色谱峰所代表的成分是造成盾叶薯蓣样品间差异的主要标志性物质,其中28、29号峰分别为盾叶新苷和三角叶薯蓣皂苷。结论本研究建立的盾叶薯蓣UPLC指纹图谱可以较为全面地表征其化学质量特征,指纹图谱的化学计量学分析结果为盾叶薯蓣质量标志物的筛选及质量标准的制定提供科学依据。

Objective To establish UPLC fingerprint of Dioscorea zingiberensis,analyze the common characteristics and differences of the quality characteristics of D.zingiberensis in different habitats,so as to provide a scientific basis for the quality evaluation of D.zingiberensis.Methods The chromatographic column was InfinityLab Poroshell 120 EC-C18(150 mm×2.1 mm,2.7μm).The mobile phase was composed of acetonitrile(B)and water(A)in gradient elution at a flow rate of 0.3 mL/min,the detection wavelength was set at 203 nm,and the column temperature was 30℃,which was used to establish the UPLC fingerprint of 65 batches of D.zingiberensis from different producing areas;SPSS 19.0 and SIMCA 14.1 software were used to evaluate the quality and analyze the differences of D.zingiberensis from different habitats.Results The UPLC fingerprint of D.zingiberensis medicinal material had marked 31 common peaks.The identified five saponin components and other unknown components all participated in the quality expression of D.zingiberensis as the main information.The comprehensive score of the load value of the main components indicated that the comprehensive quality of D.zingiberensis medicinal material from different origins was quite different;The results of PCA showed that the chemical quality characteristics of D.zingiberensis from different habitats were different,and each grouped into one category.Among them,there were significant differences between D.zingiberensis from Danjiangkou region of Hubei Province and samples from other habitats.The components represented by the peaks 5,28,11,12,29,18,16,31 and 13 selected by the PLS-DA model were the main marker substances causing the difference between the samples of D.zingiberensis,among which No.28 and No.29 peaks were zingiberensis newsaponin and deltonin.Conclusion The UPLC fingerprint of D.zingiberensis established in this study can relatively characterize its chemical quality characteristics,and the chemometric analysis results of the fingerprint provide scientific basis for screening quality markers of D.zingiberensis and establishing quality standard.