草莓白化相关病毒中国分离物全基因组分析

Genome Sequence of Strawberry Pallidosis-associated Virus Isolated in China

草莓白化相关病毒(strawberrypallidosis-associatedvirus,SPa V)属于长线形病毒科(Closteroviridae)毛形病毒属(Crinivirus),可引起草莓病害,2017年在中国首次报道。采用高通量测序、RACE和RT-PCR技术获得了SPa V中国分离物(FJ)的基因组全长。该病毒含有两条正单链基因组RNA1和RNA2。RNA1全长8 048 nt,5′和3′非编码区序列分别为264和197 nt,含有3个开放阅读框(ORF),分别编码ORF 1a/1b融合蛋白和p9蛋白。RNA2全长7 977 nt,5′和3′非编码区序列分别为248和186 nt,含有8个开放阅读框(ORF),分别编码HSP70h、CPh、CP、CPm、p7、p6、p9和p28等8个蛋白。RNA1和RNA2与美国M1分离物分别具有98.5%和99.0%的核苷酸一致性;系统发育分析结果表明,SPa V中国分离物(FJ)单独处在一个分支。对SPa V来源的小RNA的分析表明,来源于SPa V的小RAN长度以21和22 nt为主。

Strawberry pallidosis-associated virus(Spa V)belongs to the genus Crinivirus of the family Closteroviridae,which causes strawberry diseases. It was first reported in China in 2017. In this study,high-throughput sequencing,RACE and RT-PCR techniques were used to obtain the full length of SPa V China isolate(FJ). The virus contains two positive-sense single,stranded genome RNA1 and RNA2,RNA1 is composed of 8 048 nt and contains three open reading frames(ORFs),encoding ORF 1 a/1 b fusion protein and p9 protein,flanked by 5′ and 3′ untranslated regions of 264 nt and 197 nt,respectively. RNA2 is composed of 7 977 nt and contains eight ORFs,encoding HSP70 h,CPh,CP,CPm and other four unknown function proteins(p7,p6,p9 and p28),flanked by 5′ and 3′ untranslated regions of 248 and 186 nt,respectively. RNA1 and RNA2 share 98.5% and 99.0% nucleotide sequence identity with that of M1 isolate(USA),respectively. Phylogenetic analysis showed that SPa V China isolate(FJ)was clustered in a separate branch. The analysis of s RNA derived from SPa V showed that the dominant sizes were 21 nt and 22 nt.