LINC01089通过调控miR-27a-3p/TET1轴抑制胰腺癌细胞的增殖和迁移

LINC01089 inhibits the proliferation and migration of pancreatic cancer cells by regulating miR-27a-3p/TET1 axis

目的:本研究旨在探究胰腺癌中长链非编码RNA 01089(LINC01089)/miR-27a-3p/TET1轴在胰腺癌进展中的作用及其机制。方法:通过GEPIA数据库分析LINC01089在胰腺癌中的表达及其与预后的相关性。使用定量实时聚合酶链反应(qRT-PCR)检测LINC01089、miR-27a-3p、TET1 mRNA在胰腺癌组织和正常组织中的表达;CCK-8、BrdU和划痕愈合实验检测LINC01089和miR-27a-3p对细胞增殖和迁移的影响。机制上,我们通过StarBase和TargetScan数据库预测、双荧光素酶报告基因验证LINC01089和miR-27a-3p、miR-27a-3p和TET1之间的调控关系。采用Western blot检测LINC01089和miR-27a-3p对TET1表达的影响。结果:GEPIA数据库显示LINC01089在胰腺癌中低表达,其低表达与患者整体生存期(overall survival,OS)和无病生存期(diease free survival,DFS)相关。本研究中,我们发现与正常组织相比,癌组织中LINC01089表达显著下调,并与患者不良预后相关;功能实验显示LINC01089抑制胰腺癌细胞的增殖和迁移。相关机制实验表明LINC01089通过吸附miR-27a-3p上调TET1的表达在胰腺癌中发挥抑制作用。结论:LINC01089通过靶向调控miR-27a-3p促进TET1的表达,从而抑制胰腺癌细胞的增殖和迁移。

Objective:To investigate the role and mechanism of long non-coding RNA 01089(LINC01089)/miR-27 a-3 p/ten-eleven translocation 1(TET1) axis in pancreatic cancer progression.Methods:We analyzed the expression of LINC01089 in pancreatic cancer and its prognosis by GEPIA database.We used quantitative real-time polymerase chain reaction(qRT-PCR) to detect the expression of LINC01089,miR-27 a-3 p,TET1 mRNA in pancreatic cancer tissues and normal tissues.CCK-8,BrdU and scratch healing experiments were used to detect the effects of LINC01089 and miR-27 a-3 p on cell proliferation and migration.Mechanistically,we used StarBase and TargetScan database,the double luciferase reporter gene experiment to predict and verify the target relationship between LINC01089 and miR-27 a-3 p,miR-27 a-3 p and TET1.The effects of LINC01089 and miR-27 a-3 p on TET1 expression were detected by Western blot.Results:The GEPIA showed that LINC01089 was underexpressed in pancreatic cancer and correlated with patients’ overall survival(OS) and diease free survival(DFS).In this study,we found that LINC01089 expression was significantly down-regulated in cancer tissues compared with normal tissues and was associated with poor prognosis of patients.Functional experiments showed that LINC01089 inhibited the proliferation and migration of pancreatic cancer cells.Relevant mechanism experiments showed that LINC01089 upregulated the expression of TET1 by adsorbing miR-27 a-3 p and played an inhibitory role in pancreatic cancer.Conclusion:LINC01089 promotes TET1 expression by targeting and regulating miR-27 a-3 p,thereby inhibiting the proliferation and migration of pancreatic cancer.