miR-497的生物信息学分析及其在肺腺癌中表达的验证

Bioinformatics analysis of miR- 497 and its expression in lung adenocarcinoma

目的:对hsa-miR-497进行靶基因和功能生物信息学分析预测,为后续深入研究其生物学功能和调控机制提供实验理论指导,并对其在肺腺癌中的表达进行初步验证。方法:利用PubMed检索miR-497相关文章,通过miRBase在线工具分析miR-497碱基序列、染色体定位等信息。应用Targetscan及miRDB两种预测工具对miR-497进行靶基因预测取其交集,应用R中clusterProfiler包进行靶基因功能富集及信号转导通路富集分析。最后应用RT-PCR定量检测miR-497在新鲜肺腺癌及癌旁组织中的表达。结果:miR-497在多物种间具有高度的保守性,功能富集分析预测的结果显示miR-497调控的靶基因集合功能主要富集于输尿管芽生长、穿膜受体蛋白激酶、肽结合、分支形态发生和膜筏等过程(P<0.05)。KEGG生物通路显著富集于调控干细胞多能性、癌、Wnt及Rap1信号通路等(P<0.001)。miR-497在肺腺癌组织中表达明显低于癌旁组织,差异有统计学意义(P<0.05)。结论:miR-497通过靶基因调控多个生物学过程及疾病通路,尤其在肿瘤方向的生物学功能值得进一步研究,miR-497在肺腺癌中低表达为研究肺腺癌的发生及发展及治疗提供了新的方向。

Objective:To predict the target genes and functional bioinformatics analysis of miR-497,and provide theoretical guidance for further research on the biological function and regulatory mechanism of miR-497.Furthermore,its expression in lung adenocarcinoma was verified.Methods:Using PubMed to retrieve the relevant articles of miR-497,analyze its base sequence,chromosome location and other information through miRBase online tool,and analyze its conservative nature.Then,targetscan and miRDB were applied to predict the intersection of the target gene of miR-497.Finally,the target gene function enrichment and signal transduction pathway enrichment analysis were performed by using the confirmed target gene,the expression of miR-497 in fresh lung cancer and adjacent tissues was quantitatively detected by RT-PCR.Results:The results showed that the miR-497 was highly conservative among multiple species. The target gene function of has-miR-497 was mainly focused on ureteric bud development,transmembrane receprot protein kinase,peptide binding,morphogenesis of a branching and membrane raft(P<0.05).KEGG biological pathway was mainly concentrated on the interaction pathway of regulation of stem cell pluripotent,pathways in cancer,Wnt and Rap1 signaling pathway(P<0.001).The expression of miR-497 in lung cancer tissues was significantly lower than that in adjacent tissues(P<0.05).Conclusion:miR-497 regulates multiple biological processes and disease pathways through target gene,especially in the direction of tumor.Lower expression of miR-497 in lung adenocarcinoma provides a new direction for predicting the occurrence,development and treatment of lung cancer.