摘要
目的:探索miR-124及AURKA对胶质瘤细胞增殖能力的影响并探索其机制。方法:通过PCR测定胶质瘤组织及非肿瘤组织中miR-124、AURKA的表达水平;将携带miR-124的脂质体miR-124 mimic、空载体miR-124转染入胶质瘤细胞系LN-229和T98G中,吸光度观察miR-124对胶质瘤细胞系LN-229和T98G增殖的影响;通过Targetscan软件预测miR-124靶基因,Luciferase报告检测miR-124与AURKA的结合位点,通过实时定量PCR方法检测转染miR-124后AURKA基因的变化。结果:与非胶质瘤组织比较,miR-124在胶质瘤组织中表达下降,MTT实验提示miR-124高表达组胶质瘤细胞增殖能力减弱;miR-124低表达组胶质瘤细胞增殖增加,差异具有统计学意义(P<0.05)。Targetscan软件提示AURKA可能为miR-124靶基因,Western blot及荧光素酶实验提示与阴性对照组相比,miR-124高表达组内AURKA表达下降,相反miR-124低表达组内AURKA表达增加。结论:miR-124在胶质瘤细胞及组织中表达下调,并且可能通过调控AURKA影响胶质瘤细胞增殖及侵袭能力。
Objective:To explore the effects of miR-124 and AURKA on the proliferation of glioma cells and explore its mechanism.Methods:The expression levels of miR-124 and AURKA in glioma tissues and non-tumor tissues were determined by PCR.Liposome miR-124 mimic carrying miR-124 and empty vector miR-124 were transfected into glioma cell line LN-229 and T98G,the effect of miR-124 on the proliferation of glioma cell lines LN-229 and T98G was observed by absorbance.The target gene of miR-124 was predicted by Targetscan software,and the binding site of miRNA-124 and AURKA was detected by Luciferase report.Real-time quantitative PCR method detects the changes of AURKA gene after miRNA-124 transfection.Results:Compared with non-glioma tissues,the expression of miR-124 was decreased in glioma tissues.MTT experiments showed that the proliferation ability of glioma cells in the high expression group of miR-124 was weakened.The proliferation ability of glioma cells in the miR-124 low expression group increased,the difference is statistically significant(P<0.05).Targetscan software suggested that AURKA may be the target gene of miR-124.Western blot and Luciferase experiments showed that compared with the negative control group,the expression of AURKA in the high expression group of miR-124 decreased,while the expression of AURKA in the low expression group of miR-124 increased.Conclusion:miR-124 is down-regulated in glioma cells and tissues,and may affect the proliferation and invasion of glioma cells by regulating AURKA.
作者
沙娅·玛哈提
迪娜·艾尼瓦尔
肖双东
刘文
王增亮
Shaya Mahati;Dina Ainiwaer;XIAO Shuangdong;LIU Wen;WANG Zengliang(First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China)
出处
《现代肿瘤医学》
CAS
北大核心
2021年第6期930-934,共5页
Journal of Modern Oncology
基金
国家自然科学基金青年科学基金项目(编号:81801232)。