期刊文献+

miR-124通过靶向调控AURKA抑制胶质瘤细胞的增殖 被引量:4

miR-124 inhibits the proliferation of glioma cells through targeted regulation of AURKA
下载PDF
导出
摘要 目的:探索miR-124及AURKA对胶质瘤细胞增殖能力的影响并探索其机制。方法:通过PCR测定胶质瘤组织及非肿瘤组织中miR-124、AURKA的表达水平;将携带miR-124的脂质体miR-124 mimic、空载体miR-124转染入胶质瘤细胞系LN-229和T98G中,吸光度观察miR-124对胶质瘤细胞系LN-229和T98G增殖的影响;通过Targetscan软件预测miR-124靶基因,Luciferase报告检测miR-124与AURKA的结合位点,通过实时定量PCR方法检测转染miR-124后AURKA基因的变化。结果:与非胶质瘤组织比较,miR-124在胶质瘤组织中表达下降,MTT实验提示miR-124高表达组胶质瘤细胞增殖能力减弱;miR-124低表达组胶质瘤细胞增殖增加,差异具有统计学意义(P<0.05)。Targetscan软件提示AURKA可能为miR-124靶基因,Western blot及荧光素酶实验提示与阴性对照组相比,miR-124高表达组内AURKA表达下降,相反miR-124低表达组内AURKA表达增加。结论:miR-124在胶质瘤细胞及组织中表达下调,并且可能通过调控AURKA影响胶质瘤细胞增殖及侵袭能力。 Objective:To explore the effects of miR-124 and AURKA on the proliferation of glioma cells and explore its mechanism.Methods:The expression levels of miR-124 and AURKA in glioma tissues and non-tumor tissues were determined by PCR.Liposome miR-124 mimic carrying miR-124 and empty vector miR-124 were transfected into glioma cell line LN-229 and T98G,the effect of miR-124 on the proliferation of glioma cell lines LN-229 and T98G was observed by absorbance.The target gene of miR-124 was predicted by Targetscan software,and the binding site of miRNA-124 and AURKA was detected by Luciferase report.Real-time quantitative PCR method detects the changes of AURKA gene after miRNA-124 transfection.Results:Compared with non-glioma tissues,the expression of miR-124 was decreased in glioma tissues.MTT experiments showed that the proliferation ability of glioma cells in the high expression group of miR-124 was weakened.The proliferation ability of glioma cells in the miR-124 low expression group increased,the difference is statistically significant(P<0.05).Targetscan software suggested that AURKA may be the target gene of miR-124.Western blot and Luciferase experiments showed that compared with the negative control group,the expression of AURKA in the high expression group of miR-124 decreased,while the expression of AURKA in the low expression group of miR-124 increased.Conclusion:miR-124 is down-regulated in glioma cells and tissues,and may affect the proliferation and invasion of glioma cells by regulating AURKA.
作者 沙娅·玛哈提 迪娜·艾尼瓦尔 肖双东 刘文 王增亮 Shaya Mahati;Dina Ainiwaer;XIAO Shuangdong;LIU Wen;WANG Zengliang(First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China)
出处 《现代肿瘤医学》 CAS 北大核心 2021年第6期930-934,共5页 Journal of Modern Oncology
基金 国家自然科学基金青年科学基金项目(编号:81801232)。
关键词 胶质瘤 miR-124 增殖 AURKA GBM miR-124 proliferation AURKA
  • 相关文献

参考文献10

二级参考文献51

  • 1陈忠平.重视胶质瘤的化学治疗[J].中国神经肿瘤杂志,2003,1(2):65-68. 被引量:11
  • 2林丽,陈忠平.细胞因子诱导的杀伤细胞体外抗胶质瘤的实验研究[J].中国神经肿瘤杂志,2003,1(2):69-72. 被引量:8
  • 3陈忠平,赛克.神经外科医生在手术切除脑肿瘤的同时还能做些什么?[J].中国神经肿瘤杂志,2003,1(3):129-133. 被引量:3
  • 4Jacobs AH, Li H, Winkeler A,et al. PET-based molecular imaging in neuroscience[J].Eur J Nucl Med Mol Imaging,2003,30:1051 - 1065.
  • 5Kleihues P, Cavenee WK. Pathology and genetics of tumours of the nervous system [M]. Lyon(France):IARC Press,2000.1-93.
  • 6Cairncross JG, Ueki K, Zlatescu MC, et al. Specific genetic predictors of chemotherapeutic response and survival in patients with anaplastic oligodendrogliomas[J]. J Natl Cancer Inst, 1998,90:1473-1479.
  • 7Harbaugh KS, Black PM. Strategies in the surgical management of malignant gliomas [J]. Semin Surg Oncol,1998,14:26-33.
  • 8Rubino GJ, Farahani K, McGill D, et al. Magnetic resonance imaging-guided neurosurgery in the magnetic fringe fields: the next step in neuronavigation[J]. Neurosurgery, 2000,46:643-654.
  • 9Gaspar LE,Zamorano LJ,Shamsa F,et al. Permanent 125iodine implants for recurrent malignant gliomas [J].Int J Radiat Oncol Biol Phys, 1999,43:977-982.
  • 10Shrieve DC, Alexander E, Black PM,et al.Treatment of patients with primary glioblastoma multiforme with standard postoperative radiotherapy and radiosurgical boost:prognostic factors and long-term outcome[J]. J Neurosurg,1999,90:72-77.

共引文献187

同被引文献38

引证文献4

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部