多毛番茄CIPK基因家族生物信息学分析及低温下功能研究

Bioinformatics Analysis of CIPK Gene Family in Solanum habrochaites and Functional Study under Low Temperature

CIPK基因家族是Ca2+介导的植物信号转导途径中的一个关键家族,在植物胁迫响应和生长中起着关键作用。本试验基于多毛番茄全基因组数据筛选出24个多毛番茄CIPK基因家族成员,系统进化分析将家族成员分为5个亚组,家族成员内部含有5个基因复制对。亚细胞定位分析表明,多毛番茄CIPK基因大多定位于细胞质;少数分布于质膜、细胞核、线粒体。共线性分析结果表明,拟南芥和栽培番茄中的CIPK基因家族中分别有14个和12个基因与多毛番茄存在共线性关系。顺式作用元件分析结果表明,多毛番茄CIPK基因家族基因含有光、激素、冷胁迫等相关的元件。实时荧光定量PCR结果表明,低温胁迫下ShCIPK2、ShCIPK17、ShCIPK19表达量显著提高,推测这3个基因可能在多毛番茄低温胁迫应答中起重要的作用。

CIPK gene family is a critical family in Ca2+mediated plant signal transduction pathway,which plays a key role in plant stress response and growth.Based on Solanum habrochaites whole genome information,this experiment screened out 24 ShCIPK gene family members,which were divided into 5 subgroups by phylogenetic analysis,and there were 5 gene duplication pairs inside of family members.Subcellular localization analysis showed that most of the ShCIPK genes were located in cytoplasm,while a few were located in plasma membrane,nucleus and mitochondria.The results of multicollinearity analysis indicated that there are 14 and 12 genes in the CIPK gene family of Arabidopsis and cultivated tomato,being collinear with Solanum habrochaites.The result of promoter cis-acting elements prediction analysis identified that ShCIPK family genes contained relevant elements responsive to light,phytohormone,and cold stresses,etc.Real time quantitative PCR analysis showed that the expression levels of ShCIPK2、ShCIPK17、ShCIPK19 were significantly enhanced under low temperature stress,suggesting that these 3 genes might play an important role in the response of Solanum habrochaites to low temperature stress.