干扰素阴道胶囊联合微波治疗宫颈炎合并高危型HPV感染对外周血Th17和Treg细胞及炎症因子的影响

Effect of interferon vaginal capsule combined with microwave therapy on Th17/Treg cells and inflammatory factors in peripheral blood of patients with cervicitis complicated with high-risk HPV infection

目的探讨干扰素阴道胶囊联合微波治疗宫颈炎合并高危型人乳头瘤病毒(HPV)感染对外周血Th17和Treg细胞及炎症因子的影响。方法随机选择2015年1月-2018年1月在本院就诊的慢性宫颈炎合并高危型HPV感染患者90例,按随机数字表法分为观察组和对照组,每组45例。对照组给予重组人干扰素α2b栓,观察组给予重组人干扰素α2b栓联合微波治疗,治疗3个月后,观察两组患者的症状改善情况、HPV转阴率、血清炎性因子水平以及Th17和Treg细胞水平。结果观察组总有效为41例,占91.1%,对照组为73.3%,观察组显著高于对照组(P<0.05)。治疗后观察组的白带量改善率、白带脓性改善率、高危型HPV转阴率均显著高于对照组(P<0.05)。治疗前两组患者的白介素-17(IL-17)、肿瘤坏死因子-β(TNF-β)、白介素-23(IL-23)水平比较,差异无统计学意义(P>0.05);治疗后两组患者的IL-17、TNF-β、IL-23水平均显著下降,且观察组下降更加显著(P<0.05)。治疗后观察组的Treg细胞、Th17细胞水平,Th17/Treg比值均较对照组低(P<0.05)。治疗前两组患者的滴虫、真菌、细菌性阴道炎、衣原体、解脲支原体感染人数比较差异无统计学意义(P>0.05),治疗后观察组的细菌性阴道炎、衣原体、解脲支原体感染显著降低,与对照组比较差异有统计学意义(P<0.05)。结论干扰素阴道胶囊联合微波治疗宫颈炎患者的治疗效果好,可显著改善患者症状,减轻体内炎症反应,增加HPV转阴率,调节Th17和Treg细胞,对预防宫颈癌有重要作用。

Objective To observe the effect of interferon vaginal capsule combined with microwave on Th17/Treg cells and inflammatory factors in peripheral blood of patients with cervicitis complicated with high-risk HPV infection.Methods Ninety patients with chronic cervicitis complicated with high-risk HPV infection treated in our hospital from January 2015 to January 2018 were randomly selected and divided into observation group or control group,with 45 cases in each group.The control group was given recombinant human interferonα2 b suppository,while the observation group was given recombinant human interferonα2 b suppository combined with microwave therapy.After 3 months of treatment,the symptom improvement,HPV negative conversion rate,serum inflammatory factors and Th17/Treg were observed.Results The total efficiency rate was 91.11%(41 cases)in the observation group vs 73.33%in the control group,with significant difference(P<0.05).After treatment,the improvement rate of leucorrhea volume,improvement rate of purulent leucorrhea and negative conversion rate of high-risk HPV in the observation group were significantly higher than those in the control group respectively(all P<0.05).There were no significant differences in the levels of interleukin-17(IL-17),tumor necrosis factor-β(TNF-β)and interleukin-23(IL-23)between the two groups before treatment(P>0.05).After treatment,the levels of IL-17,TNF-βand IL-23 in the two groups significantly decreased,and the decrease in the observation group were more significant(P<0.05).The Treg cells,Th17 cells and Th17/Treg in the observation group were significantly smaller than those in the control group(P<0.05).There were no significant differences in the numbers of patients infected by trichomonas,fungi,bacterial vaginitis,chlamydia and Ureaplasma urealyticum between the two groups before treatment(P>0.05).After treatment,the number of patients infected with bacterial vaginitis,chlamydia and Ureaplasma urealyticum in the observation group was significantly lower than that in the control group(P<0.05).Conclusion Interferon vaginal capsule combined with microwave therapy is effective in patients with cervicitis,which can significantly improve the symptoms of patients,alleviate inflammation in vivo,increase the negative conversion rate of HPV,regulate Th17/Treg cells,and plays an important role in the prevention of cervical cancer.