基于RNA-seq技术的青藏高原不同海拔水毛茛转录组测序及基因表达分析

Transcriptome sequencing and expression analysis of Batrachium bungei(Steud.)L.Liou at different altitudes based on RNA-seq in the Qinghai-Tibet Plateau

以青藏高原不同海拔7个居群的水毛茛(Batrachium bungei(Steud.)L.Liou)为材料,对其进行转录组测序及基因表达分析,研究它们在极端环境下基因表达方面的适应性。结果显示,7个居群内样本间的基因表达具有较高的相似性。差异基因富集分析结果发现苯丙烷生物合成相关基因在5个差异组中均呈显著富集;此外类黄酮、类胡萝卜素、苯丙氨酸、酪氨酸、色氨酸的生物合成以及激素的信号转导、MAPK信号通路、植物与病原菌互作等相关基因大多被显著富集。与低海拔居群相比,参与类黄酮生物合成通路的相关基因(HHT1、HCT、F3′H、CHS、CYP73A、CCOAOMT5、CYP98A)在高海拔居群中均显著上调表达。研究结果表明水毛茛主要通过多途径的参与及关键基因的调控表达来适应青藏高原的高海拔环境。

We explored how Batrachium bungei(Steud.)L.Liou has adapted to extreme high-altitude environments.We used transcriptome sequencing and bioinformatics analysis of seven populations of B.bungei(ML,MZ,WQ,YH,YBJ,XB,ZR).Based on Pearson correlation analysis and principal component analysis(PCA),results showed that the gene expression of each population was highly similar.Differential gene enrichment analysis showed that phenylpropanoid biosynthesis was significantly enriched in five different groups.In addition,flavonoid biosynthesis,carotenoid biosynthesis,phenylalanine tyrosine and tryptophan biosynthesis,plant hormone signaling transduction,MAPK signaling pathway,and plant-pathogen interaction were significantly enriched.Compared with low-altitude populations,flavonoid biosynthesis genes(HHT1,HCT,F3′H,CHS,CYP73A,CCOAOMT5,CYP98A)were significantly up-regulated in the high-altitude populations,and their expression levels were significantly higher than those in the low-altitude(MZ)population.These results indicate that B.bungei adapted to the high-altitude environment of the Qinghai-Tibet Plateau mainly through multi-pathway participation and the regulation and expression of key genes.