摘要
蛋白质在植物细胞内的定位是了解蛋白质功能、基因调控和蛋白质-蛋白质相互作用的关键。近年来随着各种蛋白质亚细胞定位方法的快速发展和技术的不断提升,蛋白质亚细胞定位实现了高通量、活体动态研究。本文总结了植物蛋白质亚细胞定位的常用技术,以及常用细胞器特异性标记的研究进展,并对此领域研究的发展前景做出了展望。
The localization of proteins in plant cells is the key to understanding protein function,gene regulation,and protein-protein interactions.In recent years,with the rapid development of various protein-subcellular localization methods and continuous improvement in technology,protein-subcellular localization has achieved high-throughput and in vivo dynamic research.In this paper,we summarize current research progress on the common techniques of plant protein subcellular localization and development of organelle-specific markers.We also present future perspectives in this research field.
作者
未丽
刘建利
Wei Li;Liu Jian-Li(College of Pastoral Agriculture Science and Technology, State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou 730020, China;National Demonstration Center for Experimental Grassland Science Education, Lanzhou University, Lanzhou 730020, China;College of Biological Sciences and Engineering, North Minzu University, Key Laboratory of Ecosystem Modeling and Application, State Ethnic Affairs Commission, Yinchuan 750021, China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2021年第1期93-101,共9页
Plant Science Journal
基金
国家自然科学基金项目(31960346)。
关键词
蛋白质
亚细胞定位
细胞器标记物
Proteins
Subcellular localization
Organelle markers
