BC-6900全自动血液细胞分析仪检测外周血有核红细胞的准确性分析

Accuracy analysis of BC-6900 automatic blood cell analyzer for detecting nucleated red blood cells in peripheral blood

目的探讨BC-6900全自动血液细胞分析仪法(简称仪器法)检测外周血有核红细胞(NRBC)的方法学特点,评价其临床应用价值。方法同时用仪器法和显微镜检查法(简称镜检法)检测100例外周血标本中的NRBC,以镜检法为金标准,比较不同方法的计数结果,并从批内精密度、稳定性、携带污染率、人机比对方面分析BC-6900全自动血液细胞分析仪检测NRBC的效果。结果仪器法检测NRBC的灵敏度为97.959%(48/49),特异度为96.078%(49/51),假阳性率为3.922%(2/51),假阴性率为2.041%(1/49)。仪器法与镜检法检测结果有极好的相关性(r=0.9731),2种检测法比较,NRBC计数结果差异无统计学意义(P>0.05)。仪器法检测NRBC的重复性好,线性范围较宽,携带污染率小,比对试验中各项目变异系数(CV)均小于实验室允许的CV。结论BC-6900全自动血液细胞分析仪检测外周血NRBC性能良好,能满足临床医生对全血检测分析的需求。

Objective To investigate the methodological characteristics of the BC-6900 automatic blood cell analyzer method(instrument method for short)for detecting nucleated red blood cells(NRBC)in peripheral blood and evaluate its clinical application value.Methods NRBC was detected in 100 cases of peripheral blood samples by both instrumental and microscopic examination method(microscopy method for short).Using the microscope method as the gold standard,the counting results of different methods were compared,and the effect of the BC-6900 automatic blood cell analyzer in detecting NRBC was analyzed from the aspects of intra-batch precision,stability,contamination rate,and human-machine comparison.Results The sensitivity of the instrumental method to detect NRBC was 97.959%(48/49),the specificity was 96.078%(49/51),the false positive rate was 3.922%(2/51),and the false negative rate was 2.041%(1/49).There was an excellent correlation between the instrumental method and the microscopy method(r=0.9731),there was no statistically significant difference in the NRBC count results between the two detection methods(P>0.05).The instrumental method had good repeatability in the detection of NRBC,with a wide linear range and low contamination rate,the coefficient of variation(CV)of each item in the comparison test was less than the CV allowed by the laboratory.Conclusion The BC-6900 automatic blood cell analyzer has good performance in detecting peripheral blood NRBC,which can meet the needs of clinicians for whole blood detection and analysis.