酵母系统表达人表皮生长因子研究进展

Advances in Expression of Human Epidermal Growth Factor in Yeast

人源表皮生长因子是一种从人体内分离出的蛋白质,具有刺激细胞生长的作用,临床用途广泛。但由于天然来源有限,化学合成成本高,利用基因工程技术生产人表皮生长因子具有很好的前景。酵母系统作为一种典型的真核表达系统,在生产异源蛋白时能进行翻译后修饰,如糖基化、形成二硫键等,并能有效地将重组蛋白分泌到细胞外,有益于下游分离提取,在生产重组人表皮生长因子的研究中备受青睐。综述了利用酵母系统表达人表皮生长因子的研究进展,包括各种酵母表达系统在其中的应用,并分别比较其优势和劣势,在此基础上对未来的研究重点进行展望。

Human epidermal growth factor(h EGF)is a kind of protein isolated from the human body.It can stimulate the cell growth and thus has a wide range of clinical applications.The use of genetic engineering to produce hEGF has great economic prospects due to its limited natural source and high cost of chemical synthesis.Yeast expression system,as a typical eukaryotic expression system,can perform post-translational modifications,such as glycosylation,disulfide bond formation,etc.,and can effectively secrete the proteins outside of the cell so that the recombinant protein can be separated from the extracellular medium easily.Therefore,yeast is especially favored in the research of producing recombinant h EGF.This article reviews the progress of producing h EGF in various yeast expression systems,including Saccharomyces cerevisiae,Pichia pastoris,Yarrowia lipolytica,etc.,and on this base,their pros and cons were compared.The future prospects of using yeast expression to produce hEGF are discussed in light of the current progress,challenges,and trends in this field.Guidelines for future studies are finally proposed.