大麦籽粒亮氨酸QTL定位

QTL Mapping of Leucine Content in Grains of Barley

为挖掘大麦籽粒亮氨酸含量QTLs,本研究以‘紫光芒裸二棱’和‘澳选3号’构建的193个重组自交系为试验材料,采用全自动氨基酸分析仪测定了群体籽粒亮氨酸含量,利用完备区间作图法定位了大麦籽粒亮氨酸含量QTL。结果表明:籽粒亮氨酸含量在‘紫光芒裸二棱’和‘澳选3号’中分别为1.70 mg/g、0.87 mg/g,重组自交系群体后代中亮氨酸含量平均值为(1.20±0.01)mg/g,变异系数为16.92%;共检测到3个与亮氨酸含量相关的QTLs,分别位于2HL(Scssr03381~Scssr07759)、4HL(HVBAMMGB84~BMAG0808)和7HL(GMS056~GBM1297)染色体上,表型贡献率分别为7.65%、12.96%、19.74%,加性效应分别为-0.07、0.06和-0.1。本研究结果为大麦籽粒亮氨酸含量QTL精细定位及高亮氨酸含量大麦品种选育提供了理论依据。

The purpose of to map QTLs for leucine content in grains of barley.Recombinant inbred lines containing193 families derived from the crossing between’Ziguang manluoerling’and’Schooner’.Grain leucine content for the populations and their parents were determined by automatic amino acid analyzer.QTL for leucine content in grains of barley mapped by complete interval mapping method.Results shown that content of leucine in the grains were 1.70 mg/g and 0.87 mg/g in’Ziguang manluoerling’and’Schooner’,respectively.The mean and variation coefficient of leucine content were(1.20±0.01)mg/g and 16.92%in recombinant inbred lines,respectively.Three QTLs for leucine content in grains of barley were mapped.Three QTLs located on chromosome 2 HL(Scssr03381~Scssr07759),4 HL(HVBAMMGB84~BMAG0808)and 7 HL(GMS056~GBM1297),respectively.Their phenotype contribution rates were 7.65%,12.96%and 19.74%,respectively.And their additive effects were-0.07,0.06 and-0.1,respectively.The results provided a theoretical basis for fine mapping QTL of leucine content in barley grains and breeding of barley varieties with high leucine content.