质外体过氧化氢对石竹玻璃化的影响

Effect of H_(2)O_(2) in Apoplast on Hyperhydricity of Dianthus chinensis L.

为明确质外体H_(2)O_(2)(hydrogen peroxide)在组培苗玻璃化发生中的作用,本研究以石竹组培苗为材料,采用离心的方法提取质外体,并通过碘量法检测质外体H_(2)O_(2)含量,探究了培养基pH值、蔗糖、6-BA(6-Benzylaminopurine)、结冷胶及乙烯等对石竹组培苗玻璃化发生以及质外体H_(2)O_(2)含量的影响。结果表明,低pH值、低蔗糖浓度、高6-BA含量、结冷胶、乙烯利、ACC(l-aminocyclopropane-l-carboxylic acid)均使石竹组培苗玻璃化率增加,并且伴随着质外体H_(2)O_(2)含量的上升。在诱导玻璃化的培养基中外源添加二苯基氯化碘盐(diphenyleneiodonium chloride,DPI)、过氧化氢酶(catalase,CAT)、维生素B6(vitamin B6,VB6)、还原型谷胱甘肽(reduced glutathione,GSH)以及抗坏血酸(ascorbic acid,AsA)都能降低组培苗玻璃化发生率和质外体中的H_(2)O_(2)含量。说明质外体H_(2)O_(2)含量与石竹组培苗玻璃化关系密切,控制质外体H_(2)O_(2)积累可以降低石竹玻璃化的发生,这为玻璃化的有效防控提供了重要的参考。

In order to clarify the role of H_(2)O_(2)(hydrogen peroxide)in apoplast on hyperhydricity of tissue culture seedlings,Dianthus chinensis plantlets were used as materials to explore the effect of pH,sucrose,6-BA(6-Benzylaminopurine),gellan gum and ethylene on the hyperhydric rate and H_(2)O_(2) content in apoplast of Dianthus chinensis L..The centrifugation method and KI(potassium iodide)were used to extract apoplastic fluids and detect H_(2)O_(2) content,respectively.The results showed that low p H,low sucrose concentration,high 6-BA content,gellan gum,ethephon and ACC(l-aminocyclopropane-l-carboxylic acid)all increased the hyperhydric rate and the apoplastic H_(2)O_(2) content of Dianthus chinensis seedlings.Exogenous addition of diphenyliodonium chloride(DPI),catalase(CAT),vitamin B6(VB6),reduced glutathione(GSH),and ascorbic acid(AsA)in hyperdricity induction medium that reduced the incidence of hyperhydric rate and the content of H_(2)O_(2) in the apoplast.It indicated that the content of H_(2)O_(2) in apoplast was closely related to the hyperdricity of Dianthus chinensis L.seedlings.Controlling the accumulation of H_(2)O_(2) in apoplast could reduce the occurrence of hyperdricity which provides an important reference for the effective prevention and control of hyperdricity.