单菌与混合菌固态生物转化大黄素的比较研究

Comparative Study on Solid-State Fermentation of Emodin by Single and Mixed Strains

目的利用单菌与混合菌对大黄素进行微生物转化的比较研究,为提高中药材中大黄素微生物转化产率提供一种选择。方法采用58株单菌、26组混合菌进行大黄素固态生物转化,固态转化条件为温度28°C,时间5天,水分80%。采用HPLC对大黄素不同单菌和混合菌下的转化产物进行定量分析,筛选出对大黄素转化率高,ω-羟基大黄素生成率高的菌株与混合菌。结果在筛选的58株菌株、26组混合菌中,YIM321801固态转化大黄素的转化率为30.7%,ω-羟基大黄素生成率22.8%;YIM3088固态转化大黄素的转化率为21.1%,ω-羟基大黄素生成率0.8%;而混合菌YIM321801:YIM3088固态转化大黄素,转化率达到60.1%;ω-羟基大黄素的生成率为32.9%。结论本研究发现混合菌YIM321801(青霉属Penicillium):YIM3088(台湾根霉Rhizopus formosensis)对大黄素转化率最显著,比单菌转化更有优势,为大黄素混合菌转化奠定一定的基础。

Objective The compare the microbial transformation of emodin by single strains and mixed strains to provide an alternative for improving the yield of microbial transformation of emodin in Chinese medicinal materials.Methods Emodin is fermented by 58 single strains and 26 mixed strains. The conditions of solid-state fermentation was28°C for 5 days with humidity levels of 80%. HPLC was used for quantitative analysis of transformation products of emodin by different single strains and mixed strains. The strains or mixed strains with high conversion rate of emodin and high productivity of ω-hydroxyemodin were selected.Results Among the 58 single strains and 26 mixed strains, the conversion rate of YIM321801 in solid-state fermentation of emodin was 30.7% with the ω-hydroxyemodin production rate of 22.8%. The conversion rate of YIM3088 in solid-state fermentation of emodin was 21.1% with the ω-hydroxyemodin production rate of 0.8%. The conversion rate of mixed strains YIM321801: YIM3088 in solid-state fermentation of emodin reached 60.1% with the ω-hydroxyemodin production rate of 32.9%. Conclusion It was found that the mixed strains YIM321801(Penicillium): YIM3088(Rhizopus formosensis) had the most significant transformation rate of emodin, which was more advantageous than the single strains, and laid a certain foundation for the transformation of emodin by mixed strains.