摘要
目的探讨Netrin-1在视网膜母细胞瘤(RB)中的表达情况以及其干预是否会影响RB对顺铂的敏感性。方法收集24例RB患者的肿瘤组织,其中19例为初治者,5例为复发者,采用Real-time PCR和Western blotting检测Netrin-1基因(在多种肿瘤中高表达)的表达情况。利用慢病毒干扰RB细胞系Y79中Netrin-1的表达,并检测细胞的增殖情况以及顺铂处理后的细胞凋亡情况。结果Netrin-1在RB患者肿瘤组织中呈高表达(P<0.05),且在复发患者中的表达水平明显高于初治者(P<0.05);干扰Netrin-1可抑制RB细胞的增殖(P<0.05),还可增加肿瘤对顺铂的敏感性(P<0.05)。结论Netrin-1可能在RB的形成和耐药机制中发挥重要作用。
Objective To investigate the expression of Netrin-1 in retinoblastoma(RB)and the effects of interfered Netrin-1 on its sensitivity to cisplatin.Methods The tumor tissues of 24 patients with retinoblastoma were collected,including 19 first-treated patients and 5 refractory patients.The expression of Netrin-1 was detected by real-time PCR and Western blotting methods.Lentivirus was used to interfere with the expression of Netrin-1 in retinoblastoma cell line Y79.The proliferation of cells was detected,and the apoptosis after cisplatin treatment was also analyzed.Results Netrin-1 was highly expressed in retinoblastoma(P<0.05),and its expression in refractory patients was higher than in firsttreated patients(P<0.05).Interference with Netrin-1 can inhibit the proliferation of retinoblastoma cells(P<0.05)and increase their sensitivity to cisplatin(P<0.05).Conclusion Netrin-1 may play an important role in the formation of retinoblastoma and drug resistance mechanism.
作者
喻一心
邓娅青
江海波
YU Yixin;DENG Yaqing;JIANG Haibo(Eye Center of Xiangya Hospital,Central South University,Changsha,Hunan,410008,China;Hunan Key Laboratory of Ophthalmology,Changsha,Hunan,410008,China;Department of Ophthalmology and Visual Sciences,the Kellogg Eye Center,University of Michigan,MI,48105,USA)
出处
《肿瘤药学》
CAS
2021年第1期31-35,共5页
Anti-Tumor Pharmacy
基金
吴阶平医学基金会临床科研专项资助项目(320.6750.18119)。
