小麦剩余杂合体株高的基因定位

BSR-seq Mapping for Plant Height Based on Sequential Residual Heterozygotes in Wheat

为挖掘小麦矮秆基因,以矮秆小麦品种品冬34和高秆小麦品种Warran杂交构建的重组自交系(RIL)群体为材料,于F7:8筛选出一个株高性状分离的剩余杂合体。利用BSR-seq技术对该剩余杂合体分离的高株与矮株纯系分别混池测序,进行株高位点的初步定位。结果表明,与株高性状相关的候选区域位于7B染色体687~689 Mb处,该区段内有18个候选基因。其中,TraesCS7B02G419700和TraesCS7B02G420400既参与代谢过程,又参与小麦茎的生长发育;而TraesCS7B02G418900、TraesCS7B02G419000、TraesCS7B02G419600和TraesCS7B02G420000虽在茎中表达,却未参与相关代谢过程。这6个基因特别是TraesCS7B02G419000和TraesCS7B02G420400可作为调控株高性状的候选基因进行深入研究。

In order to explore new dwarf genes of wheat,a RIL population was constructed by crossing Pindong 34 and Warran,and one pairs of the residual heterozygotes line(RHL)with significant difference in plant height were selected from F7:8 RIL populations.The BSR-seq was used to locate the dwarf gene preliminarily by mixed pool sequencing from high and dwarf homozygous lines,respectively.The results showed that the candidate region associated with plant height was mapped at 687-689 Mb on chromosome 7B,in which 18 candidate genes were annotated by wheat URGI database.Among them,TraesCS7B02G419700 and TraesCS7B02G420400 are involved in metabolic process and stem development.Besides,TraesCS7B02G418900,TraesCS7B02G419000,TraesCS7B02G419600 and TraesCS7B02G420000 were also involved in wheat stem development,but they did not participate in metabolic process by KEGG.Therefore,the six genes can be further studied as candidate genes for regulating the trait of plant height,especially TraesCS7B02G419000 and TraesCS7B02G420400.The preliminary mapping and functional analysis can provide a theoretical basis for the cloning of wheat dwarf genes and their application in wheat breeding in the future.