神经肽Y在DNFB诱发的ACD慢性瘙痒模型小鼠背根神经节中的表达

Expression of Neuropeptide Y in Dorsal Root Ganglion of Mice with Allergic Contact Dermatitis Induced by DNFB

目的利用2,4二硝基氟苯(DNFB)诱发C57BL/6小鼠建立过敏性接触性慢性瘙痒模型(ACD)。检测神经递质神经肽Y(NPY)等与瘙痒有关的基因在背根神经节(DRG)中的表达,并探讨其对ACD小鼠模型中伴发慢性瘙痒的作用。方法将8~12周龄的C57BL/6雄性小鼠随机分成两组,分别为DNFB诱导的实验组和单独使用丙酮的对照组。通过观察小鼠的搔抓行为学、分析皮肤的炎症状况、利用HE染色和免疫荧光技术分析皮肤的病理改变和肥大细胞浸润情况、定量PCR分析NPY、MRGPRA3、TRPA1、TRPV1、TRPM8在颈部背根神经节(DRG)的mRNA水平的表达。结果DNFB诱发的ACD模型组小鼠的搔抓行为显著多于丙酮对照组。同对照组相比,ACD模型组小鼠皮肤表现出明显的炎性皮损,皮损组织表现为表皮增厚,真皮中肥大细胞浸润增加,在颈背部背根神经节中NPY mRNA水平显著增高。结论DNFB会诱发小鼠产生过敏性接触性慢性瘙痒和加剧炎性皮损的表现,NPY在ACD慢性瘙痒中有重要作用。

Objective To establish an allergic contact dermatitis model(ACD)in C57BL/6 mice induced by 2,4 dinitrofluorobenzene(DNFB)and detect the expression of itch related genes such as neurotransmitter neuropeptide Y(NPY)in dorsal root ganglion(DRG).Methods The C57BL/6 mice,8~12 weeks old,were randomly divided into two groups,a DNFB-induced experiment group and an acetone control group.The behavior of mice and the inflammation of the skin were observed.Skin histology and mast cell infiltration were analyzed by HE staining and immunofluorescence,and quantitative PCR analysis of mRNA expression changes of NPY,MRGPRA3,TRPA1,TRPV1,TRPM8 in the cervical dorsal root ganglion(DRG).Results The spontaneous scratches of ACD model mice was significantly increased than the control group.Compared with the control group,the skin of the ACD model group showed obvious inflammatory skin lesions,skin pathology showed epidermal thickening,mast cell infiltration,and increased NPY mRNA expression level in the cervical and dorsal ganglion.Conclusion DNFB can induce allergic contact chronic itching and promote the promotion of skin inflammation.NPY plays an important role in the ACD chronic itching.