摘要
目的·探讨脐静脉内皮细胞外泌体对炎症刺激下小鼠前软骨细胞ATDC5凋亡的影响。方法·采用试剂盒分离人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)外泌体,采用Western blotting检测外泌体标志蛋白肿瘤易感基因101(tumor susceptibility gene 101,Tsg101)、白细胞分化抗原9(cluster differentiation 9,CD9)、凋亡相关基因2互作蛋白X(apoptosis linked gene-2-interacting protein X,Alix)的表达水平。透射电镜观察外泌体形态,粒度检测鉴定外泌体大小。使用荧光显微镜观察外泌体被小鼠前软骨细胞ATDC5摄取过程和活性氧(reactive oxygen species,ROS)的生成情况。TUNEL染色和流式细胞术检测外泌体对白细胞介素-1β(interleukin-1β,IL-1β)刺激下的ATDC5细胞凋亡的影响。Western blotting检测外泌体对于ATDC5细胞中凋亡相关蛋白B淋巴细胞瘤2(B-cell lymphoma/leukemia-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、裂解的半胱天冬酶3(cleaved caspase-3,c-caspase-3)和抗氧化应激相关蛋白核因子E2相关因子2(nuclear factor E2 related factor 2,Nrf-2)、Kelch样环氧氯丙烷相关蛋白1(Kelch-like ECH-associated protein 1,Keap-1)、血红素加氧酶1(heme oxygenase 1,HO-1)、还原型烟酰胺腺嘌呤二核苷酸醌氧化还原酶1(NADPH quinone oxidoreductase-like protein 1,NQO-1)表达水平的影响。结果·透射电镜下观察到HUVEC来源的外泌体呈椭圆形,中空,双层膜,并且阳性表达外泌体标志物CD9、Alix、Tsg101。与IL-1β刺激下的ATDC5细胞相比,外泌体促进炎症因子作用下的ATDC5细胞内ROS的生成(P=0.000)和凋亡的发生(P=0.000),Bax、c-caspase-3、Keap-1表达升高,Bcl-2、Nrf-2、HO-1、NQO-1表达降低。结论·HUVEC来源的外泌体可能通过抑制ATDC5细胞抗氧化应激的能力,促进IL-1β刺激下ATDC5细胞凋亡的发生。
Objective·To investigate the effect of exosomes derived from umbilical vein endothelial cells(HUVECs)on apoptosis of murine prechondrogenic cell line ATDC5 cells under inflammatory stimulation.Methods·The exosomes derived from HUVECs were isolated by using an exosome isolation kit.Western blotting was used to detect the exosome marker proteins,including tumor susceptibility gene 101(Tsg101),cluster differentiation 9(CD9)and apoptosis linked gene-2-interacting protein X(Alix).The morphology of exosomes was observed by transmission electron microscope,and the size of exosomes was identified by particle size detection.Fluorescence microscope was used to observe the ATDC5 cell uptake of exosomes and the production of reactive oxygen species(ROS).TUNEL staining and flow cytometry were used to examine the effect of exosomes on ATDC5 cell apoptosis stimulated by interleukin-1β(IL-1β).Western blotting was used to detect the effect of exosomes on the expression levels of ATDC5 apoptosis-related proteins such as B-cell lymphoma/leukemia 2(Bcl-2),Bcl-2 associated X protein(Bax),cleaved caspase-3(c-caspase-3)and anti-oxidative stress-related proteins such as nuclear factor E2 related factor 2(Nrf-2),Kelch-like ECH-associated protein 1(Keap-1),heme oxygenase 1(HO-1)and NADPH quinone oxidoreductase-like protein 1(NQO-1)under IL-1βstimulation.Results·Under the transmission electron microscope,the HUVEC-derived exosomes were oval,hollow,double-layered,and positively expressed exosome markers CD9,Alix and Tsg101.Compared with the ATDC5 cells stimulated by IL-1β,ATDC5 cells stimulated by IL-1βincubated with exosomes had higher level of ROS(P=0.000)and higher apoptosis rate(P=0.000).The expression of Bax,c-caspase-3 and Keap-1 increased,and the expression of Bcl-2,Nrf-2,HO-1 and NQO-1 decreased in ATDC5 cells exposed to IL-1βand exosomes compared to ATDC5 cells only exposed to IL-1β.Conclusion·HUVEC-derived exosomes may promote ATDC5 cells apoptosis under the stimulation of IL-1βby inhibiting the ability of ATDC5 cell to resist oxidative stress.
作者
杨润泽
许文宁
郑火亮
蒋盛旦
YANG Run-ze;XU Wen-ning;ZHENG Huo-liang;JIANG Sheng-dan(Department of Clinic of Spine Center,Xinhua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China)
出处
《上海交通大学学报(医学版)》
CAS
CSCD
北大核心
2021年第2期147-153,共7页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金面上项目(81672206)
上海市教育委员会高峰高原学科建设计划(20181809)。
关键词
外泌体
骨关节炎
前软骨细胞
脐静脉内皮细胞
氧化应激
凋亡
exosome
osteoarthritis(OA)
pre-chondrogenic cell
human umbilical vein endothelial cell(HUVEC)
oxidative stress
apoptosis