岩藻多糖通过SIRT1/Nrf2抑制高脂饮食诱导神经前体细胞衰老的研究

A Study of Fucoidan Inhibiting the Premature Senescence of Neural Precursor Cells Induced by High-fat Diet through SIRT1/Nrf2

目的研究岩藻多糖(fucoidan)抑制高脂饮食诱导神经前体细胞衰老的作用及机制。方法选用C17.2细胞作为神经前体细胞的模型细胞,根据不同的刺激条件分为对照组、棕榈酸刺激组、棕榈酸+岩藻多糖组,刺激72h。观察各组的β-半乳糖苷酶(β-galactosidase activity,β-gal)染色情况,细胞内活性氧(reactive oxygen species, ROS)水平,p21、p53、SIRT1蛋白表达情况。根据观察结果初探岩藻多糖抑制棕榈酸诱导C17.2细胞衰老的机制。增加SIRT1和Nrf2抑制剂组,观察β-gal染色、ROS水平、p21、p53、Nrf2、Nqo1蛋白表达情况。根据观察结果,验证SIRT1/Nrf2在岩藻多糖抑制棕榈酸诱导C17.2细胞衰老过程中的作用。结果与棕榈酸刺激组比较,棕榈酸+岩藻多糖组细胞β-gal染色阳性率和衰老相关蛋白p53、p21表达显著降低(P<0.05)。SIRT1、Nrf2、Nqo1的蛋白表达水平以及Nrf2的入核水平均明显增加(P<0.05)。加入SIRT1抑制剂NAM后,Nrf2和Nqo1蛋白表达、Nrf2入核水平显著降低(P<0.05),ROS水平明显增加(P<0.05)。加入NAM或Trigonelline后,C17.2细胞的衰老表型明显增加(P<0.05)。结论岩藻多糖可抑制高脂饮食诱导的神经前体细胞衰老,这一过程可能与激活SIRT1/Nrf2抑制细胞氧化应激有关。

Objective To study the effect and mechnism of Fucoidan on inhibiting the high-fat diet(HFD)-induced senescence of Neural precursor cells(NPCs).Methods C17.2 cells were selected as the model cells of NPCsand they were divided into control group,palmitic acid(PA)stimulation group and PA+Fucoidan group according to different stimulation conditions,and stimulated for 72h.We observed theβ-galactosidase(β-gal)staining of each group,the level of reactive oxygen species(ROS)in the cells and the expression of p21,p53,SIRT1 protein.Based on the observation results,the effect of fucoidan′sinhibition on HFD-induced aging of NPCs was explored.Then we added SIRT1 and Nrf2 inhibitor respectively to observeβ-gal staining,ROS level and p21,p53,Nrf2,Nqo1 expression.According to the observation results,the role of SIRT1/Nrf2 in Fucoidan′s inhibition of HFD-induced NPCs senescence was veritified.Results Compared with the HFD group,the positive rate ofβ-gal staning and expression of p53,p21 significantly decresed in PA+Fucoidan Group(P<0.05).Furthermore,the expression of SIRT1,Nrf2,Nqo1 and the entry level of Nrf2 into nuclear increased significantly(P<0.05).After adding SIRT1 inhibitor(NAM),the expression of Nrf2,Nqo1 and level of Nrf2 into nuclear decreased significantly(P<0.05)and the level ROS increased significantly(P<0.05).By the way,adding NAM and Trigonelline both rasiedthe aging characteristics of C17.2 cells(P<0.05).Conclusion Fucoidan can inhibit the senescence of NPCs induced by high-fat diet.And this process may be related to the activation of SIRT1/Nrf2 to inhibit the oxidative stress of C17.2 cells.