丹酚酸对ADP诱导的血小板聚集的影响

Inhibitory Effects of Salvianolic Acids on ADP-induced Platelet Aggregation

目的研究丹酚酸(salvianolic acid,SA)及其代谢物对腺苷-5'二磷酸(adenosine-5-diphosphate,ADP)诱导的血小板聚集的影响,为寻找到高效低毒的抗血栓药物提供依据。方法取健康雄性SD大鼠动脉血浆,以药物替格瑞洛为阳性对照,ADP为诱导剂,考察SA对血小板聚集的体外作用,用比浊法测定血小板聚集率,结果用IC_(50)表示。结果 SA表现出中等的体外抑制ADP诱导血小板聚集的作用,IC_(50)值0.3~0.9 mmol/L。结论丹酚酸具有温和的抑制体外ADP诱导血小板聚集的作用,且有很好的研究价值。

Objective To investigate the role of salvianolic acids(SA)and the metabolites on ADP-induced platelet aggregation for the development of antithrombotic drugs with high efficiency and low toxicity.Methods Arterial plasma was collected from healthy male SD rats.Taking Ticagrelor as positive control,the in vitro role of salvianolic acids on ADP-induced platelet aggregation was examined by turbidimetry and the results were expressed by IC_(50).Results Salvianolic acids displayed moderate inhibitory effect on ADP-induced platelet aggregation with IC_(50) values ranging from 0.3 to 0.9 mmol/L.Conclusion Salvianolic acids have gentle inhibitory effect on ADP-induced platelet aggregation in vitro,indicating superior research value.